An experiment was carried out to evaluate the chemical active ingredients present in Superliv®, a liquid herbal mixture produced by Ayurvet India and marketed in Nigeria by Animal Care Konsult, using the essential oil method involving the extraction process, the gas chromatography and the mass selective detector (MSD) technique. Results from this study shows that the major active ingredient in the herbal supplement is Pyrimidine (83.33%) which has been considered as an effective antioxidant. The other part of the experiment assessed the In-vitro antimicrobial effect of Superliv®. Theherbal supplement (Superliv®) was incorporated into MacConkey agar medium which was prepared by suspending 47g in 1 litre of distilled water. Dissolved completely and then sterilized by autoclaving at 121oC for 15minutes. After cooling to about 55oC, it was poured into Petri-dish and sensitive Escherichia coli and Staphylococcus aureus were inoculated into the medium and incubated. The herbal supplement had no significant antimicrobial in-vitro effect on the gram positive and gram negative bacteria at the manufacturers recommended dosage. However at an increased concentration of 60 -100% it had inhibitory growth effect on the gram positive and while at 90-100% on negative bacteria.
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