RANDOM AMPLIFICATION POLYMORPHIC DNA, A GOOD EPIDEMIOLOGIC SCREENING METHOD FOR NOSOCOMIAL ISOLATES OF PSEUDOMOS AERUGINOSA.

Abstract

Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens. To characterizes P. aeruginosa strains that are widespread in patients in Hilla city,300 clinical and environment samples were collected from wounds, burn, ear, stool ,nose ,sputum and urinary tract infection taken from general hospitals of Hilla city. Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, were used in this study. The results show that, the selective medium (cetrimide agar) at 42˚C aerobically had highest recovery in the isolation of P. aeruginosa, they were produced greenish-yellow or blue pigment colonies, catalase and oxidase was positive whereas negative for methyl red, VogesProskauer and indole. A total of 34 amplified DNA fragmentsfrom 250 to 1500 bp)were observed using the 6 random primers,Amplification bands were exclusively revealed with four out of the six random primers (OPB-10,OPX-01,272,275) While( RAPD TYPING, 325) primers were failure to give amplification bands, and each of primer that successful giving amplification bands revealed different genetic patterns .

Conclusion:RAPD-PCR analysis proved to be of great value in designing a variety of molecular based epidemiological studies that focuses on the identification and characterization of P.aeuroginosa

 

 

Keywords: PCR, Pesudomonas aeruginosa, RAPD, nosocomial

Article Review Status: Published

Pages: 37-44 (Download PDF)

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