The aim of this study was to conduct an assessment of the knowledge of mobile digital devices in the learning of Chemistry in Nigeria tertiary institutions. To achieve this purpose, three research questions were generated, these are: what is the percentage of Chemistry students who have access to mobile digital devices in Nigeria tertiary institutions?; to what extent have mobile digital devices improve the teaching and learning of Chemistry in Nigeria tertiary institutions?; does the use of mobile digital devices motivate students to learn Chemistry in Nigeria tertiary institutions?. This study adopted descriptive survey design. Stratified random sampling method was used to select 300 students from Osun state tertiary institutions, data was collected using checklists. Data generated were analyzed using frequency counts, simple percentages and relative importance index. The findings of this study revealed that minority of students have access to the following digital mobile devices; smart phones, notebooks, internet, while majority of students don’t have access to the following digital devices: desktop computer, laptops, touch screen tablets, I pad, cameras, recorders and android devices. Based on the findings of this study, recommendations were made.