Histomorphological Effects of Aqueous Leaf Extract Of Phyllanthus Amarus Shum and Thonn on the Testis of Adult Wistar Rats (Published)
The study was conducted for twelve weeks to investigate the effects of 250 mg/kg body weight of ethanolic leaf-extract of Phyllanthus amarus on the morphology and sperm parameters of the testis. Twenty (20) adult male Wistar rats were categorized into two groups of ten rats each (n=10) with A (control), B (to assess the effects of the extract on testicular morphology and sperm parameters). The body and organ weight of the experimental animals were noted before and at the end of the experiment and the significance of data was analyzed using student t- test (p<0.05). The results revealed no significant difference (p>0.05) in the body and organ weight between the experimental groups and the control. There were significant difference (p<0.05) in the total sperm count, sperm motility and viability in the treatment group compared to control with obvious decrease in all the parameters. The rats treated with ethanolic extract of P.amarus showed various degrees of testicular degeneration compared to the control demonstrating seminiferous tubular lumen with scanty spermatozoa, distorted seminiferous epithelium and arrested differentiation and progression of germ cells into the lumen. The epididymis also showed scanty and moderate clusters of spermatozoa in the lumen with thickened epithelial lining. The findings are consistent with anti-fertility remarks on the effects of P. amarus on testicular morphology and functions.
Toxicity Impacts Of Silk Dye Waste on Sperm Parameters and Histopathology of Testis of Swiss Albino Male Mice Mus Musculus and Its Mitigation by Using Moringa Oleifera Leaf Extract (Published)
The environmental toxicology studies the effect of environmental toxicant on the health of organisms and on the different compartments of the environments. Different scientific studies have been carried out over the years on the toxic effect of the silk dyes waste effluents on organisms. But current study has been premeditated to evaluate the effect of Moringa oleifera (Lam) leaf extract on albino male mice Mus musculas which is subjected to silk dye waste. The sperm profile and histopathology of testis have been taken on accounts. Five sets of animals i.e. Group I (Control), Group II (fed with 50% silk dye), Group III (fed with 100% silk dye), Group IV (mice fed with 50% dye treated with M. oleifera leaves powder), Group V (mice fed with 100% dye treated with M. oleifera leaves powder) have been taken for experiment. The dose of silk dye was 2ml/day to both groups II and III and M. oleifera leaf is given as per the standard dose (300mg/kg b.w) to both animals of group IV and V. The results show that the M. oleifera leaf extract when fed to Gr IV and V mice, demonstrated the regeneration of germinal epithelial cells and basement membrane, significant mitigation of spermatogenesis in different stage of testis when compared with mice of Gr- II and III. Then the M. Oleifera leaf increased significantly number of sperm count and % of sperm motility but decrease the % of sperm mortality of mice when compared with Gr-II and III at 5% and 10% level of probability. This study suggested that the extract may have beneficial effect on histological sections of testis and sperm count, mortality and motility.