Tag Archives: Sequencing

A Discourse Analysis of Selected Football Texts (Published)

This study investigates the discourse analysis of football texts. The choice of football texts from the Nigerian newspaper was motivated by my personal interest in football and the popularity soccer has gained in recent years across the world. The study covers the football texts selected from Complete Sports which dominated the Nigerian sports’ news market. The largest number of sports lovers across the country source their information on sports most especially football news from the selected newspaper based on its popularity and accurate reporting of sporting activities the world over. Lead article from each of the newspaper were selected for a period of one month during 2010 world cup making a total of 30 lead articles. Ansary and Babai 2005 discourse Generic Structure Potential was applied to unmask the discourse structure of football text in newspapers and it contextual configuration. Talking about the discourse Generic Structure Potential (GSP), it is the total structural resources of text genre. This organizational sequencing are classified as obligatory, optional and recursive elements. Each of these classifications serves specific purposes or functional role in the texts.

Keywords: Discourse, Football, Generic structure, Genre, Sequencing, structural resources

Molecular Characterization of Hepatitis C Virus Strain Circulated In Chronically Infected Patients in Abidjan (Cote-D’ivoire) (Published)

Viral hepatitis C (HCV) is a public health problem. The therapeutic management and in particular the duration of treatment depends on the viral genotype. HCV is poorly documented in the population and there are few data on the different genotypes and subtypes of HCV circulating in Côte d’Ivoire. In this context, the main objective of this study was to study the genetic variability of the HCV virus in infected patients in Abidjan. We conducted a cross-sectional study at CIRBA from June 2015 to June 2017 which included adult patients with a Viral Load > 1000 IU/mL. HCV genotyping was performed by amplification of the NS5B region followed by sequencing with an ABI 3130 sequencer (Applied Biosystems, Courtaboeuf, France). Phylogenetic trees were produced using MEGA 7 software and genotypes were confirmed using online software (http://hcv.geno2pheno.org). In this study 94 subjects were included. The genotypes encountered were genotypes 1, 2 and 4 with a prevalence of 46%, 52% and 2% respectively. These strains were divided into 17 subtypes genotype 1 : 6 subtypes 1a, 1b, 1c, 1d, 1i, 1k, genotype 2 : 9 subtypes 2a, 2b, 2c, 2c/k, 2f, 2j, 2k, 2l, 2r and 2 subtypes 4f and 4r for genotype 4. The study allowed the implementation of a genotyping technique and monitoring showed that genotypes 1 and 2 are predominant in Côte d’Ivoire. The circulation of genotype 4 is noted.

Keywords: HCV, Sequencing, Treatment., genotype.