This study is aiming at assessing the quality of work life for academic staff in Jordanian universities. And to identify the possibility of the existence of a statistical significant difference in their attitudes as it relates to quality of work life, which can be attributed to the variables of gender, age, academic rank, and experience. Academic staff were asked to fill questionnaire assessing the following QWL indicators: fair compensation, safe and healthy environment, work-life balance, social integration and career advancement towards university. The research most important findings and conclusions are: The arithmetic mean of the estimates of academic staff in the Jordanian universities towards quality of work life were moderate. Besides, there were no statistical significant on quality work life the due to variables (gender, age, academic qualification, experience).