Tag Archives: Punica Granatum

Phytochemical Constituents and Antioxidant Properties of Methanolic Leaf Extract of Punica Granatum L. (Published)

Phytochemical constituent and antioxidant properties of the methanolic leaf extract of Punica granatum was investigated. Extracts of the leaf was subjected to quantitative and qualitative phytochemical screening, In vivo and In vitro scavenging activity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) were determined. The free radical scavenging activity of DPPH and H2O2 have SC50 of 1.069±0.29µmol/ml and 1.819±0.29µmol/ml respectively. DPPH actitvity of these extract shows no significant difference when compared with a standard value of Vitamin C is 1.140±0.26µmol/ml whereas H2O2 shows significant difference as compared to standared Vitamin C with SC50 value of 1.331±0.31µmol/ml. The administration of the extract at 200mg/kg and 400mg/kg body weights of wistar albino rats significantly increased the levels of catalase 39.02±0.72nmol/mg and 39.55±0.62nmol/mg respectively with a negative control of 7.53±1.94nmol/mg as compared to the normal control value of 37.76±0.63nmol/mg whereas lipid peroxidation level decreased significantly at treatment groups 200mg/kg and 400mg/kg with value of 4.96±0.20(nmol/mg)/10-6 and 4.35±0.17(nmol/mg)/10-6 respectively when compared to the negative control group with a value of 8.21±0.15(nmol/mg)/10-6 and normal control value of 4.36±1.67(nmol/mg)/10-6, although there is no significant difference as compared to the normal control groups. These findings suggest possible antioxidant properties of the methanolic leaf extract of Pomegranate

Keywords: Antioxidant activity, CCL4, DPPH, Oxidative stress, Punica Granatum, free radicals scavenging activity

Identification and Characterization of the Phenolic Profile of A Hydroalcoholic Extract of Punica Granatum (Published)

More than half the world’s population use or used at some point a herbal because of its potential healing. The pomegranate, as well as another 70 medicinal plants are listed as species of interest to the public health system in order to generate new effective and safe medicines. The objective of this study was to evaluate a hydroalcoholic extract of Punica granatum about their biochemical and biophysical characteristics. The extract was serially diluted and exposed to different conditions. The values of wavelengths, absorbance, pH and conductivity were observed. The analysis values, it can be concluded that the extract in question has the characteristic of stability in the different conditions and that has possibly flavonoids, tannins and anthocyanins in its composition.

Keywords: Anthocyanins., Biochemistry, Biophysics, Characterization, Flavonoids, Punica Granatum, Tannins