Antibacterial Effect of Gongronema Latifolium Leaf Extracts On Selected Gram Positive and Negative Clinical Bacterial Isolates (Published)
This work was aimed at assaying the in-vitro effect of aqueous and ethanolic leaf extracts of Gongronema latifolium on Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli. Six (6) milimetre sterile discs were impregnated with the aqueous and ethanolic extracts at different concentrations ranging from 6.25mg/mL to 100mg/mL. The test organisms were spread evenly on Mueller Hinton agar plate and the discs were aseptically placed on them. The sensitivity plates were incubated at 37ºC for 24 hours. All the test organisms showed sensitivity to both aqueous and ethanolic leaf extracts of Gongronema latifolium. The zones of inhibition were concentration dependent, ranging from 2.0mm to 10.8mm for aqueous extract and 2.0mm to 8.3mm for the ethanolic extract. Comparison of the zones of inhibition produced by the two extracts showed that there is no statistical difference (P > 0.05) between aqueous and ethanolic extracts. Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae had Minimum inhibitory concentration (MIC) of 6.25mg/mL, while Pseudomonas aeruginosa had MIC of 25mg/mL for the aqueous extract. The MIC was 3.125mg/mL, 6.25mg/mL, 6.25mg/mL and 25mg/mL for S. aureus, E. coli, K. pneumoniae and P. aeruginosa, respectively for the ethanolic extract. Gongronema latifolium extracts were also bactericidal in action. S. aureus, E. coli and P. aeruginosa all had Minimum bactericidal concentration (MBC) of 6.25mg/mL, while K. pneumoniae had MBC of 25mg/mL for the aqueous extract, while for the ethanolic extract, S. aureus, E. coli, K. pneumoniae and P. aeruginosa had MBC of 12.5mg/mL, 12.5mg/mL, 6.25mg/mL and 3.125mg/mL respectively. The data obtained from the study indicated that both the aqueous and ethanolic leaf extracts of Gongronema latifolium possess antibacterial properties. Therefore, the pharmaceutical industries should consider its usage for the production of novel antibiotics.
Phyto-Microbial Degradation of Glyphosate in Riyadh Area (Review Completed - Accepted)
Greenhouse studies were conducted to determine the ability of plant Amaranth, Amaranthus caudate and two isolated bacterial strains from rhizosphere region for cleaning up glyphosate residues in soil and plants. The analytical study of the biodegradation of glyphosate was carried out in the laboratory conditions. Amaranth, Amaranthus caudate and two isolated bacterial strains namely Pseudomonas aeruginosa and Bacillus megaterium could degrade glyphosate in 5 days. These results suggested that phytoremediation could accelerate the degradation of glyphosate residues in plants and in rhizosphere region as well. Glyphosate had strong effect on bacterial DNA where many DNA bands were affected. This could be explained that the effect of herbicide glyphosate on the protein profile may reflex somehow DNA mutation occurred during the assimilation of those toxic compounds. Therefore, the alteration occurred in both DNA and protein profiles is considered a degree of tolerance that lead to DNA mutation to cope with the assimilation of this compound. Therefore, the phytoremediation way could be a promising tool in program is to protect public health and the environment by ensuring the safety and availability of herbicides and pesticide alternatives.