Evaluating In Vivo Antiplasmodial Potency of Aqueous Extract of Phyllanthus Niruri in Swiss Albino Mice (Published)
In vivo antiplasmodial potency of aqueous leaf extract of phallanthus niruri used in malaria naturopathy, a global public health scourge was evaluated in unsexed 100 4-week old, 5 groups of 20 swiss albino mice each weighing 18-22g passaged with rodent plasmodium berghei NK-65 except Sham. Control/Sham received distilled water. Other 3 groups received 200, 400; 800mg/kg of extract respectively and focally/histologically sampled for 7 consecutive days (d). After 7d, Control exhibited high density load of parasitemia, marked lethargy, white eyelids and extreme hemodilution compared to treated groups. % residual parasitic density with time (d) were 35 + 4% (200mg/kg), 13 + 1% (400mg/kg), 10 + 1% (800mg/kg) compared to 65 + 7% (Control) with Ct½ 1.4 < 2.3 < 4.8 < 7d : 800 < 400 < 200mmkg < Control (p < 0.05). No adverse renal or hepatic effects. These results strongly demonstrate a potent safe in vivo antiplasmodial activity of P. niruri.
Keywords: antiplasmodial activity, aqueous leaf extract, phyllanthus niruri, swiss albino mice.
Phytochemical Screening, Proximate and Mineral Compositional Analyses of Phyllanthus Niruri Leaves (Published)
Potent antimalarial effectiveness of aqueous leaf extract of P. niruri plant was recently demonstrated in this laboratory. As a prelude to detailed mechanistic studies of this aqueous leaf extract, an in-house phytochemical, proximate and mineral compositional analyses of P. niruri leaf samples, by AOAC procedures were hereto undertaken. Results of phytochemical screening of the leaf samples revealed the presence of alkaloids, tanins, antraquinones, glycosides, saponins, flavonoids, steroids and terpenoids. Proximate analysis showed percentage values of moisture, ash, crude fibre, ether extract, crude protein, nitrogen free extract and carbohydrate contents. Mineral compositional analysis revealed the presence (mg/l) of major and minor elements, Zn, Fe, Na, Mn, K, Mg, Ca, and Cu, in natural blend. These results show potentials of P. niruri leaf samples and suggesting it as a good source for mineral supplementation. These findings may also explain some basis of antimalarial properties of aqueous extract of P. niruri leaves.
Keywords: Phytochemical screening, aqueous leaf extract, phyllanthus niruri, proximate and mineral analyses
Evaluating in Vivo Antiplasmodial Potency of Aqueous Leaf Extract of Phyllanthus Niruri in Swiss Albino Mice (Published)
In vivo antiplasmodial potency of aqueous leaf extract of Phallanthus niruri used in malaria naturopathy, a global public health scourge was evaluated in unsexed 100 4-week old, 5 groups of 20 swiss albino mice each weighing 18-22g passaged with rodent Plasmodium berghei NK-65 except Sham. Control/Sham received distilled water. Other 3 groups received 200, 400; 800mg/kg of extract respectively and focally/histologically sampled for 7 consecutive days (d). After 7d, Control exhibited high density load of parasitemia, marked lethargy, white eyelids and extreme hemodilution compared to treated groups. % residual parasitic density with time (d) were 35 + 4% (200mg/kg), 13 + 1% (400mg/kg), 10 + 1% (800mg/kg) compared to 65 + 7% (Control) with Ct½ 1.4 < 2.3 < 4.8 < 7d : 800 < 400 < 200mmkg < Control (p < 0.05). No adverse renal or hepatic effects. These results strongly demonstrate a potent safe in vivo antiplasmodial activity of P. niruri.
Keywords: antiplasmodial activity, aqueous leaf extract, phyllanthus niruri, swiss albino mice.
