Tag Archives: mycelium

Possible Domestication of Edible Wild Mushroom Agaricus silvaticus G. J. Keizer In Rivers State, Nigeria (Published)

The experiment on the artificial cultivation of Agaricus silvaticus was conducted in crop protection laboratory at the Rivers State University. Wild edible mushroom fruiting bodies used were collected from three different sites (site one, site two, site three) in Ogwe, Ukwa West Local Government Area, Abia State. Mycelium (basidia) production was significantly achieved and cultivated in a modified ancient Indian traditional method, site two (66.0) significantly had the highest mycelium production compared to site three (49.0) and site one (48.0). The mycelium from 3 sites was used to inoculate the spent grain to achieved spawn production. Site two recorded the highest spawn production (56.0) followed by site one (52.0) and least at site three (39.0). Germination of Agaricus silvaticus pin-heads (primordia) were achieved by inoculating the spawn from the three sites into decomposed palm bunch (DPB) and decomposed saw dust (DSD) for fruiting bodies determination. The experimental results therefore shows significant difference on the relationship between two substrates decomposed palm bunch (DPB) in site one having the highest (53.7) followed by site two (46.3) and site three (41.3) and no growth in decomposed saw dust (DSD). It is note-worthy that this experiment successfully achieved mycelium production, spawn production and cropping of Agaricus silvaticus. This is a major scientific breakthrough and first trial for the cultivation of Agaricus silvaticus in Rivers State and Nigeria at large. Thus, the knowledge will contribute to the commercial production of Agaricus mushroom which is a dietary delicacy in most African Countries though completely dependent on seasonal growth from the wild.         

Keywords: Agaricus silvaticus, mycelium, spawn and pin-head germination

Isolation and Identification of some Fungi and Bacteria in soils Colonized by Edible Wild Mushroom Agaricus silvaticus G. J. KEIZER in Rivers State, Nigeria. (Published)

This research on the isolated and morphologically identification of fungi and bacteria in soils naturally colonized by wild mushroom Agaricus silverticus was carried out in the crop protection laboratory at the Rivers State University.  Soil samples were collected from 4 different locations in Ogwe, Ukwa West Local Government Area, Abia State, Nigeria: 3 sample sites from Obiahia kindred the mushroom colonized soils in its natural habitats and the fourth a control plot from Obiawom kindred where mushroom was not found. The experimental result significantly (P˂0.05) revealed the presence of six fungi genera: four mushroom inhibiting microbes namely Penicillium spp., Sclerotium spp., Mucor spp. and Aspergillus spp. in decreasing order from the control sites where Agaricus mushroom was not found and two other benefiting fungi genara: Yeast and Fusarium spp. from the Agaricus Mushroom colonized soils. However, fairly insignificant number (P>0.05) of Penicillium spp. was also recorded in site three of the Agaricus colonized soil habitat though overshadowed by the very high presence of Yeast. Similarly, six bacterial genera (Bacillus spp, Proteus spp, Micrococcus spp, Streptococcus spp, Staphylococcus spp and Pseudomonas spp) were isolated and morphologically identified. It is note-worthy that Pseudomonas and Bacillus spp. which enhances mycelia development and promote primordial differentiation were significantly present in Agaricus colonized soil samples and absent in control soils. This is the first trial report for the microbial assay of Agaricus silvaticus in Rivers State and Nigeria at large and will contribute to the artificial cultivation and management of edible wild mushroom Agaricus spp which is a dietary delicacy in Southern Nigeria and most African Countries.

Keywords: Agaricus silvaticus, fungi and bacteria in soil samples, mycelium, spawnand pin-head.