The purpose of this study is to determine the presence of bacteria and other microorganisms in cosmetic samples, brought in by female students of the College of Health Sciences, and to identify these bacteria by microbiological analysis. A total of 100 expired, commercial cosmetic products, were provided by college students. They include: Mascaras, lipsticks, lip-glosses, eye shadows, eye-liners, foundation creams, body creams and talk powders. The products were tested for the presence of microorganisms by using general, enrichments and selective media. Microscopic examination and biochemical analysis (Catalase test and API test) were then performed for isolated pure cultures. 22% of the expired cosmetic products were contaminated to various degrees. Two types of yeasts were isolated: Candida Albicans, found in compact powder with a contamination rate of 20%, eye shadows, 6.6% and foundation creams, 10%. Rhodotorula yeast was isolated from eye liner with a contamination rate of 6.6%. In addition, three species of staphylococci were isolated: S. xylosus, S. warneri and S. schleiferi. Other bacterial species, like Micrococcus, Streptococcus and Bacillus species, were also isolated. These species are present normally on human skin as a normal micro-flora However, some of these microorganisms, known as opportunistic pathogens, may cause skin infection and irritation, especially, when sharing these products with others, as the micro-flora may vary from one person to another.
Citation :Jenan M. Al- Rifaaia, Aneesah M. Al Haddad, Yousef. S. N. Alrefaei (2021) Types of Bacteria Found in Cosmetics Used by Female College Students in Kuwait, European Journal of Biology and Medical Science Research, Vol.9, No.4, pp.20-33
Assessment of Student Awareness and Knowledge about Contaminated mobiles at colleges in Kuwait (Published)
In this era, most people of all ages possess a mobile phone; these mobiles are essential gadgets that became significantly advanced in technology and are convenient for communication, making life easier globally. The aim of this study is to investigate and assist the students’ Awareness and Knowledge about Contaminated mobiles at colleges in Kuwait. A self- administered questionnaire was distributed among 150 college students that were randomly selected and were asked to answer the questionnaire genuinely. These college students were from different departments such as; Medical laboratory, dental, natural science, nutrition, the department of pharmacology and nursing college. The questionnaire was analyzed statistically using SPSS versionV26. Results show that college students had a lack of awareness, knowledge attitude and behavior towards contaminated mobiles and the transmission of pathogens.
Evaluation of the Pharmacological Activities of Beta-Sitosterol Isolated From the Bark of Sarcocephalus Latifolius (Smith Bruce) (Published)
Beta Sitosterol was previously isolated from the bark of Sarcocephalus latifolius (smith Bruce). This was subjected to antimicrobial screening with the following microorganisms to determine its pharmacological activities; Methycilin Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus pyrogenes, Bacillus subtilis, Corynebacterium ulcerans, Escherichia coli, proteus mirabilis, proteus vulgaris, Pseudomonas aureginosa, Salmonella typhi, Shigellia dysenteric, Candida albicans, Candida virusei, and Candida tropicalis. The zone of inhibition was observed to be between 20 to 27 mm with Bacillus subtilis and Salmonella typhi to be the most sensitive organism with the highest zones of inhibition of 27mm. The Beta Sitosterol was found to inhibit Staphylococcus aureus, Streptococcus pyrogenes, Bacillus subtilis, Corynebacterium ulcerans, vulgaris, Pseudomonas aureginosa, Salmonella typhi, Shigellia dysenteric, Candida albicans and Candida virusei at 12.5µg/ml. While the MBC/MFC was minimum for Bacillus subtilis ,Salmonella typhi and Shigellia dysenteric at 25µg/ml, and Staphylococcus aureus, Streptococcus pyrogene, Corynebacterium ulcerans, Proteus vulgaris, Proteus aureginosa, Candida albicans and Candida virusei were at 50µg/ml. The result further shows that it could be compared with a standard drug.