Tag Archives: Malondialdehyde

Assessment of the Heavy Metals and Natural Radioactivity in Phosphate Mines and Occupational health effects at some Egyptian Regions (Published)

In this work the specific activities of natural radionuclides namely (238U series, 232Th series and 40K) has been measured in collected sedimentary phosphate deposits samples from El-Hamraween, El-Quser and Safaga phosphate mines in Egypt. HPGe γ-spectrometry were used. This study was undertaken to estimate the radiation hazard indices in phosphate mining at the studied mines on their occupational workers, and to establish correlation relationships between the some measured heavy metals such as As, Cd and Pb in blood workers and their concentration in phosphate rock ores. As well as determination the biomarkers in the blood workers such as malondialdehyde (MDA) and superoxide dismutase (SOD). The phosphate mine sample of El-Hamrawein has the lowest activity concentration of 226Ra, 232Th and 40K in compared to the phosphate mine samples of El-Quseir and Safaga (El-Hamrawein<El-Quseir<Safaga). The activity concentrations of all analyzed investigated radionuclides considerably fluctuated; for 226Ra activity concentrations varied from 222.4 to 255.8 Bq kg1, 122.4 to 188.3Bq kg1and 115.4 to 165.8Bq kg1 for Safaga,El-Quseir and El-Hamrawein, respectively. For 232Th activity concentrations varied from 135.6 to 212.3 Bq kg1, 112.8 to 167.4Bq kg1and 132.8 to 188.6 Bq kg1 for Safaga, El-Quseir and El-Hamrawein, respectively. For 40K activity concentrations varied from 225.2 to 312.8 Bq kg1, 168.7 to 268.9Bq kg1and 95.2 to 155.8Bq kg1 for Safaga, El-Quseir and El-Hamrawein, respectively. The workers of old ages have higher concentration of the investigated heavy metals than young ages. There is a good relation between the concentration of the investigated metals in phosphate mine samples and their concentration in blood of the occupational workers in these mines.

 

Keywords: Heavy Metals, Malondialdehyde, Natural radionuclides, occupational workers. Health effects, phosphate, superoxide dismutase

The Effect Of Green Tea on Oxidative Stress Level Among Wistar Suplemented By Recycling Canola Oil (Published)

Oxidative stress is involved in aging and many diseases, such as neurodegenerative, psychiatric disorders, and various cancers. Recyling oil contains trans fat (TF), one of sources of oxidative stress induce lipid peroxidation resulting in malondialdehyde (MDA) and 4-hydroxynonenal (HNE) generation. Green tea has gained considerable attention because of its antioxidant features. This study focuses on the effect of green tea on plasma MDA levels as one of marker for oxidative stress among wistar fed by recycling canola oil. A post test only control group design study using wistar rat. Samples were randomly divided into five groups (n=5 per group). K1 (negative control), K2 was given recycling canola oil and standard diet for 4 weeks, K3 was given green tea and standard diet for 4 weeks, P1 was given green tea and recycling canola oil diet for 4 weeks and P2 was given recycling canola oil diet for 4 weeks then green tea and standard diet for 4 weeks. Blood samples were collected from abdominal aorta to measure plasma MDA levels using modification of TBARS methods as described by Yagi et al.

There is no significant difference between groups on body weight (p=0.310), abdominal circumference (p=0.503) and plasma MDA levels (p=0.398) after giving green tea to wistar fed by standard diet and supplemented by recycling canola oil. We conclude that giving green tea to wistar fed by standard diet and suplemented by recycling canola oil do not influence on body weight, abdominal circumference and plasma MDA levels.

 

Keywords: Malondialdehyde, Oxidative stress, Trans fat