Chronic Hyperglycemia and Biochemical Alterations Induced Hepatic-Renal Dysfunction in Rats Administered Ethylacetate Fraction of Plumbago Zeylanica, Linn (Published)
Chronic hyperglycemia is an independent predictor of death in myocardial infarctions and nephropathy and it is a major cause of organ damage. Previous studies have shown that administration of Plumbago zeylanica extract elevated significantly the glucose concentration of the organism even though there have been many claims of the ameliorative effects of the plant. Hence, we investigated the likely dysfunctions of liver and kidney that may results from the induced hyperglycemia. Twenty rats (150-190 g) were grouped into four of five rats per group. Group 1 (control) was administered 2% Tween-20 and groups 2, 3 and 4 were administered 100, 200 and 400 mg/kg b. wt. doses of P. zeylanica ethylacetate (PZE) fraction respectively. Administration of PZE for 28 days significantly increased the plasma glucose level suggesting hyperglycemia. The activities of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly increased thereby suggesting hepatic dysfunction and the kidney function monitored by plasma protein, creatinine and urea levels was impaired by PZE suggesting renal dysfunction. However, there was no significant change in the level of lipid profile. PZE elicited hyperglycemic effect, hepatic and renal dysfunctions in a dose-dependent manner. Thus, the inducement of hyperglycemia by PZE may have resulted into organ damage noticed in the liver and kidney. Therefore, PZE should be used as herbal medicine with caution.
Concern for hyperglycemia due to diabetes mellitus and its complications in male diabetics prompted the need for this study. Making use of body fluids such as urine, seminal fluid, serum sample and excised tissues, we examined factors that can potentiate complications. Batteries of tests conducted includes insulin, glucose, glycated haemoglobin, urea, creatinine and some male sex hormones, FSH, LH and testosterone. Spectrophotometric, radioimmunoassay and histopathological approach were adopted for the analytical methods. A total of 100 subjects were studied out of which 50 were non diabetics as defined by their glucose concentration (3.5-5.6 mmol) and 50 diabetics with glucose concentration ranging from (15.5-35.5 mmol/l). Our findings portray a correlation among the factors and elicit a nexus that interrelate to cause complication in diabetes. Values of the analyte measured after a 24 hour fast showed a significant difference (p<0.05) between the diabetics and non diabetics while no statistical significant difference was observed among the normal subjects. We submit that variation in the values of biochemical parameters measured and their interactive interplay act in uncommon way to exercerbate diabetic complications and reduce fertility in males. Inhibiting these factors highlighted from rising will improve fertility among male diabetics.