Tag Archives: Hepatotoxicity

Hepatoprotective Activity of Aqueous and Methanolic Extracts of Watermelon Seeds On Salt and Paracetamol-Induced Liver Damage in Female White Rats (Published)

Paracetamol is the commonest over the counter drug for the treatment of body pains. However, an overdose of this drug has detrimental effects on the liver and other internal organs such as the kidneys. This experiment was conducted to investigate the hepatoprotective and regenerative effects of aqueous and methanolic extracts of watermelon seeds on the liver of rats given overdose of common salt and paracetamol. Thirty five (35) female albino rats were used for this experiment and the rats were randomly distributed into seven groups with each group containing five (5) rats. The rats in group A were given only vital feed (40% of the body weights of the rats) without any extract. This was the normal group. The rats in groups B, D and E were given physiological saline at the dosage of 10g/kg body weight once orally. In addition, 200 mg/kg and 400 mg/kg (body weight) of aqueous extract of watermelon seed were given to rats in groups D and E orally twice daily respectively. The rats in groups C, F and G were given paracetamol at dosage of 3g/kg body weight once orally at the beginning of the experiment. In addition, 200 mg/kg and 400 mg/kg (body weight) of methanolic extracts of watermelon were given to rats in groups F and G orally twice daily respectively. These experiments were monitored for 28 days. The results of the histopathological analyses showed that overdose of salt and paracetamol damaged the liver cells of the rats. However, treatments of the rats with aqueous and methanolic extracts of the seeds of watermelon have hepatoprotective and regenerative properties.

Keywords: Cirrhosis, Citrullus Lanatus, Extracts, Hepatoprotective, Hepatotoxicity, Liver, Necrosis, Paracetamol, histopathology

Protective Effect of Garlic Oil Alone or Combined With Low-Dose Gamma Irradiation on Paracetamol-Induced Hepatotoxicity in Rats (Published)

We investigated the effect of garlic oil (GO), alone or combined with low dose total body gamma (γ)-irradiation (LDR) against paracetamol (APAP)-induced hepatotoxicity in rats. GO (100 mg/kg/day) was administered orally for 14 days before hepatotoxicity induction. Animals were irradiated 2 hours before hepatotoxicity induction by peroral APAP (1g/kg) administration, then animals were sacrificed 24 hours later. GO, alone or combined with LDR, ameliorated APAP-induced adverse effects as revealed by the reduction of serum liver marker enzymes activities and the histological examination. Hepatic microsomal cytochrome P2E1 activity was also reduced. Changes in hepatic redox balance were significantly attenuated by both treatments. Hepatic hydrogen peroxide was only reduced by LDR. APAP-induced mitochondrial dysfunction and apoptotic effect were also reversed. GO and/or LDR pretreatment, effectively protected against APAP-induced hepatotoxicity. Thus, the hepatoprotective effect of GO alone or combined with LDR could be of value in the protective management of APAP-induced hepatic damage.

Keywords: Apoptosis, Garlic, Hepatotoxicity, Low Dose (Γ)-Radiation, Mitochondrial Dysfunction, Oxidative stress

PROTECTIVE EFFECT OF GARLIC OIL ALONE OR COMBINED WITH LOW-DOSE GAMMA IRRADIATION ON PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS (Published)

We investigated the effect of garlic oil (GO), alone or combined with low dose total body gamma (γ)-irradiation (LDR) against paracetamol (APAP)-induced hepatotoxicity in rats. GO (100 mg/kg/day) was administered orally for 14 days before hepatotoxicity induction.Animals were irradiated 2 hours before hepatotoxicity induction by peroral APAP (1g/kg) administration, then animals were sacrificed 24 hours later.GO, alone or combined with LDR, ameliorated APAP-induced adverse effects as revealed bythe reduction ofserumliver marker enzymes activities and the histological examination. Hepatic microsomal cytochrome P2E1 activity was also reduced. Changes in hepatic redox balance were significantly attenuated by both treatments. Hepatic hydrogen peroxide was only reduced by LDR. APAP-induced mitochondrial dysfunction and apoptotic effect were also reversed. GO and/or LDR pretreatment, effectively protected against APAP-induced hepatotoxicity. Thus, the hepatoprotective effect of GO alone or combined with LDR could be of value in the protective management of APAP-induced hepatic damage

Keywords: Apoptosis, Garlic, Hepatotoxicity, Low Dose (Γ)-Radiation, Mitochondrial Dysfunction, Oxidative stress