Cytomegalovirus IgM Antibody Detection and Hepatitis B and C Coinfections among HIV Patients in Ado-Ekiti, Nigeria (Published)
Opportunistic viral infections such as Cytomegalovirus (CMV), Hepatitis B (HBV) and C (HCV) are often prevalent in HIV patients undergoing antiretroviral therapy (ART) thereby shortening the life span of infected patients. The co-infection of CMV, HBV and HCV among HIV positive patients attending ART clinics across hospitals in Ekiti State, Nigeria, were studied. Ninety-two (92) blood samples from HIV positive individuals (males, pregnant females and non-pregnant females) were collected and serologically screened for CMV antibodies (IgM) using enzyme linked immunosorbent assay (ELISA) while HBV and HCV surface antigen were determined using standard test kit. The viral loads were determined using COBAS® AmpliPrep / COBAS® TaqMan® systems. The occurrence of single or coinfection of HIV-patients with respect to their sampling class were also determined and expressed in percentage. Of samples screened, the total positive occurrence rates of 23.16±1.78%, 11.20±1.46% and 30.21±2.67% were observed for CMV, HBV and HCV while 35.43±1.85% of total respondents tested negative to all the viruses screened in this study. The total viral loads were 10184 copies/ml for CMV, 5679 copies/ml for HBV and 12678 copies/ml for HCV. For the coinfections, respondents with HBV+HCV had a total occurrence of 31.80±2.25% (13294 copies/ml) while HCV+CMV had total occurrence of 44.40±3.18% (51783 copies/ml), CMV+HBV had total occurrence of 19.25±1.67% (8679 copies/ml) and CMV+HCV+HBV had a total occurrence of 9.50±1.35% (586 copies/ml). This high prevalence rate of CMV with Hepatitis co-infection among these HIV positive individuals further support the role of viral reactivation in immunocompromised patients.
Incidences of Hepatitis B Surface Antigen and Hepatitis C Antibodies in Diabetics Patient at Oouth (Published)
In recent time, controversy have been stirred up over the aetiology of liver failure in diabetic patients. Hence, this study was carried out to determine the incidence of Hapatitis B surface antigen and Hapatitis C antibodies in diabetic patients at OOUTH. 5ml venous blood samples was collected from the diabetic patients, centrifuged at 1500 rpm for 5minutes and screened for HBV and HCV using ELISA and Diaspot Kits respectively. Out of the 230 diabetic patients screened for Hepatitis Virus Infection, 49 (19.6%) of them were positive while 185 (80.4%) were negative. HBV infection was highest in frequency by 43 (95.6%), followed by HBV and HCV co-infection, with a frequency of 2 (4.4%). Gender and ages of diabetic patients were observed not to have significant relationship with the incidence of viral hepatitis infection (P>0.05). Incidence of viral hepatitis was significantly higher in uneducated and widowed diabetic patients (P<0.05). Higher incidence of viral hepatitis infection was found to be significantly associated with tattooing (P<0.05) while no significant association was observed between same infection and each of alcoholism, blood transfusion, previous surgery and number of sex partners (P>0.05). When the diagnostic test result of diaspot was compared with ELISA, sensitivity of 11.1% and 82.2% were respectively observed (P<0.05).In conclusion, this study has established that the incidence of viral hepatitis was higher among diabetic patients with no formal education, those who are widows, and tattoo. It was further discovered that diaspot is less sensitive compared to ELISA