Clinical and biochemical profiles of TP53 codon 249 mutation in Senegalese patients with hepatocellular carcinoma (HCC) (Published)
Hepatocellular carcinoma (HCC) is a public health issue. It is developed mainly on the bed of chronic liver diseases dominated in our contexts by carriage of chronic HBV and exposure to aflatoxin (AFB1). The aim of our study was to determine the prevalence of codons 248 and 249 mutations of TP53 gene and to study the clinical and biochemical profiles of patients with HCC in Senegal.DNA was extracted from paraffin blocks and fresh tissue from patients diagnosed with HCC. An exon 7 amplification step was followed by digestion (RLFP) with the enzymes Hae III, and Msp I which cut respectively the GG / CC fragments of codon 249 and C / CGG of codon 248.A total of 27 patients with a mean age of 43.7 were included in the study. Males were predominant with a sex ratio of 5.75. Codon 249 mutation was found in 6 patients (22.1%). 5 of them had heterozygous profile. No mutation was found on codon 248. Mutation of codon 249 was specifically found in patients with chronic HBV. Patients with codon 249 mutation were twice as likely to have an altered general condition at the time of diagnosis (RR (CI) = 2.1 (0.5-8C4)) with hyperbilirubinemia (RR = 3.4 (0.4-26.7)). Mutation was more encountered in patients with a large tumour (greater than 10 cm), localized at the right side.Our study confirms hypothetical specificity of codon 249 mutation of exon 7 of TP53 gene in hepatic carcinogenesis in patients with chronic HBV. It seems to be a good molecular marker for the prevention of this cancer in this population at risk.
Cytomegalovirus IgM Antibody Detection and Hepatitis B and C Coinfections among HIV Patients in Ado-Ekiti, Nigeria (Published)
Opportunistic viral infections such as Cytomegalovirus (CMV), Hepatitis B (HBV) and C (HCV) are often prevalent in HIV patients undergoing antiretroviral therapy (ART) thereby shortening the life span of infected patients. The co-infection of CMV, HBV and HCV among HIV positive patients attending ART clinics across hospitals in Ekiti State, Nigeria, were studied. Ninety-two (92) blood samples from HIV positive individuals (males, pregnant females and non-pregnant females) were collected and serologically screened for CMV antibodies (IgM) using enzyme linked immunosorbent assay (ELISA) while HBV and HCV surface antigen were determined using standard test kit. The viral loads were determined using COBAS® AmpliPrep / COBAS® TaqMan® systems. The occurrence of single or coinfection of HIV-patients with respect to their sampling class were also determined and expressed in percentage. Of samples screened, the total positive occurrence rates of 23.16±1.78%, 11.20±1.46% and 30.21±2.67% were observed for CMV, HBV and HCV while 35.43±1.85% of total respondents tested negative to all the viruses screened in this study. The total viral loads were 10184 copies/ml for CMV, 5679 copies/ml for HBV and 12678 copies/ml for HCV. For the coinfections, respondents with HBV+HCV had a total occurrence of 31.80±2.25% (13294 copies/ml) while HCV+CMV had total occurrence of 44.40±3.18% (51783 copies/ml), CMV+HBV had total occurrence of 19.25±1.67% (8679 copies/ml) and CMV+HCV+HBV had a total occurrence of 9.50±1.35% (586 copies/ml). This high prevalence rate of CMV with Hepatitis co-infection among these HIV positive individuals further support the role of viral reactivation in immunocompromised patients.
Incidences of Hepatitis B Surface Antigen and Hepatitis C Antibodies in Diabetics Patient at Oouth (Published)
In recent time, controversy have been stirred up over the aetiology of liver failure in diabetic patients. Hence, this study was carried out to determine the incidence of Hapatitis B surface antigen and Hapatitis C antibodies in diabetic patients at OOUTH. 5ml venous blood samples was collected from the diabetic patients, centrifuged at 1500 rpm for 5minutes and screened for HBV and HCV using ELISA and Diaspot Kits respectively. Out of the 230 diabetic patients screened for Hepatitis Virus Infection, 49 (19.6%) of them were positive while 185 (80.4%) were negative. HBV infection was highest in frequency by 43 (95.6%), followed by HBV and HCV co-infection, with a frequency of 2 (4.4%). Gender and ages of diabetic patients were observed not to have significant relationship with the incidence of viral hepatitis infection (P>0.05). Incidence of viral hepatitis was significantly higher in uneducated and widowed diabetic patients (P<0.05). Higher incidence of viral hepatitis infection was found to be significantly associated with tattooing (P<0.05) while no significant association was observed between same infection and each of alcoholism, blood transfusion, previous surgery and number of sex partners (P>0.05). When the diagnostic test result of diaspot was compared with ELISA, sensitivity of 11.1% and 82.2% were respectively observed (P<0.05).In conclusion, this study has established that the incidence of viral hepatitis was higher among diabetic patients with no formal education, those who are widows, and tattoo. It was further discovered that diaspot is less sensitive compared to ELISA