Tag Archives: genotype.

Molecular Characterization of Hepatitis C Virus Strain Circulated In Chronically Infected Patients in Abidjan (Cote-D’ivoire) (Published)

Viral hepatitis C (HCV) is a public health problem. The therapeutic management and in particular the duration of treatment depends on the viral genotype. HCV is poorly documented in the population and there are few data on the different genotypes and subtypes of HCV circulating in Côte d’Ivoire. In this context, the main objective of this study was to study the genetic variability of the HCV virus in infected patients in Abidjan. We conducted a cross-sectional study at CIRBA from June 2015 to June 2017 which included adult patients with a Viral Load > 1000 IU/mL. HCV genotyping was performed by amplification of the NS5B region followed by sequencing with an ABI 3130 sequencer (Applied Biosystems, Courtaboeuf, France). Phylogenetic trees were produced using MEGA 7 software and genotypes were confirmed using online software (http://hcv.geno2pheno.org). In this study 94 subjects were included. The genotypes encountered were genotypes 1, 2 and 4 with a prevalence of 46%, 52% and 2% respectively. These strains were divided into 17 subtypes genotype 1 : 6 subtypes 1a, 1b, 1c, 1d, 1i, 1k, genotype 2 : 9 subtypes 2a, 2b, 2c, 2c/k, 2f, 2j, 2k, 2l, 2r and 2 subtypes 4f and 4r for genotype 4. The study allowed the implementation of a genotyping technique and monitoring showed that genotypes 1 and 2 are predominant in Côte d’Ivoire. The circulation of genotype 4 is noted.

Keywords: HCV, Sequencing, Treatment., genotype.

Effect of the Environment on the Adaptability of Biofortified Bean Genotypes in the Eastern Democratic Republic Of Congo: Case of South Kivu (Published)

Faced with climatic, soil deterioration and technical constraints, bean production is low and very variable in space and time. The selection of the best performing genotypes is faced with the presence of significant interaction between genotype x environment which significantly reduces effectiveness. An experiment following a randomized complete block design was conducted during two cultural seasons B2012 and B2013 in 3 edapho-climatic characteristic sites in eastern Democratic Republic of Congo: Luvungi, Kashusha and Bitese and aimed to analyze the interaction genotype x environment yield of six biofortify bean genotypes, in order to identify the best in these ecosystems. Germination rate, day number to the flowering stage, days number to the physiological maturity stage, harvested plants number, pods per plant number, number of seeds per pod, weight of 100 seeds were observed. The interaction was analyzed using AMMI model and typological with by the Chord similarity indices. On one hand, genotypes with specific adaptability, namely Maharagi Kalanga in Luvungi; CodMLB001 in Kashusha and BRB194 and RWK10 in Bitese. On other hand, those broad adaptations as HM21-7 for Kashusha and Luvungi and RWR2245 for Kashusha and Bitese were also highlighted. Popularization and positive exploitation the interaction on high production potential sites and the general adaptation on relatively lower potential sites improves yield by valorizing each environment and the adoption of these genotypes by farmers.

Keywords: Biofortified bean, Bitese, Kashusha, Luvungi, Selection, genotype.