Tag Archives: Garlic

Protective Effect of Garlic Oil Alone or Combined With Low-Dose Gamma Irradiation on Paracetamol-Induced Hepatotoxicity in Rats (Published)

We investigated the effect of garlic oil (GO), alone or combined with low dose total body gamma (γ)-irradiation (LDR) against paracetamol (APAP)-induced hepatotoxicity in rats. GO (100 mg/kg/day) was administered orally for 14 days before hepatotoxicity induction. Animals were irradiated 2 hours before hepatotoxicity induction by peroral APAP (1g/kg) administration, then animals were sacrificed 24 hours later. GO, alone or combined with LDR, ameliorated APAP-induced adverse effects as revealed by the reduction of serum liver marker enzymes activities and the histological examination. Hepatic microsomal cytochrome P2E1 activity was also reduced. Changes in hepatic redox balance were significantly attenuated by both treatments. Hepatic hydrogen peroxide was only reduced by LDR. APAP-induced mitochondrial dysfunction and apoptotic effect were also reversed. GO and/or LDR pretreatment, effectively protected against APAP-induced hepatotoxicity. Thus, the hepatoprotective effect of GO alone or combined with LDR could be of value in the protective management of APAP-induced hepatic damage.

Keywords: Apoptosis, Garlic, Hepatotoxicity, Low Dose (Γ)-Radiation, Mitochondrial Dysfunction, Oxidative stress

DIAGNOSIS OF SOME TYPES OF YEASTS ASSOCIATED WITH THE HUMAN BODY AND TREATED IN THE GARLIC AND COLOCYNTHIS EXTRACTS (Published)

97 swabs were collected from the vagina, the mouth, middle ear, skin and urine of clinical cases for patients with candidiasis disease in Diyala province for the period of July 2010 – February 2011 for the diagnosis of some types of yeasts associated with the human body and treated with garlic and colocynthis extracts. The results of diagnosis showed that Candida albicans and C.tropicalis yeasts associated to the human body by 69.35% and 13.3%. colocynthis extract showed cellular toxicity of red blood cells. Hexane extracts of garlic and colocynthis gave highest percentages 52% and 63%, followed Alcoholic extracts 50% and 45%, then the hot and cold water extracts 33%,35% 0.25% and 30%, respectively,alcohol and hexane extracts of garlic and colocynthis showed inhibition effectiveness higher than in cold and hot water extracts.The inhibition effectiveness of garlic extracts better than colocynthis extracts against C. albicans and C.tropicalis yeast, hexane extract of garlic at 100 mg/ ml gave higher inhibition percentages 100% against C.tropicalis, followed by alcoholic extract 98.88% against C. albicans and C.tropicalis, and the lowest inhibition percentages 57% at 20 mg / ml for the cold water extract of the garlic plant too. The results showed that MIC of Fluconazole 12.5 and 6.5 µg / ml against C.albicans and C.tropicalis , and then Ketacanazole and Nystation 25 and 12.5 µg / ml respectively . Whereas MFC of antifungal 50 µg / ml. hexane extraction for garlic and colocynthis at 100 mg / ml have been showed inhibition effectiveness equalized to inhibition effectiveness for antifungal of Nystatin at 2 mg/mI

Keywords: Antifungal, Colocynthis, Garlic, culture media and patients

EFFECT OF INTRA-ROW SPACING ON GROWTH PERFORMANCE OF GARLIC (ALLIUM SATIVUM) AT THE EXPERIMENTAL SITE OF WOLLO UNIVERSITY, SOUTH WOLLO, ETHIOPIA (Published)

A field experiment was conducted at the experimental site of Wollo University, kelemeda during in 2013 cropping season. The objective of this study was to determine the effect of intra-row spacing on the growth performance of garlic. The experiment was conducted on a randomized complete block design with three replications. Only one local variety was used on the experiment. The result of the experiment revealed significant difference among treatments with regard to plant height at 56 days after emergence. Plant height was influenced by intra row spacing such that plant height increases when the intra row spacing of the plant decreases. A significance difference was also recorded in leaf width, leaf length and leaf number per plant as influenced by intra row spacing (P<0.05). The highest leaf width (1.99) was recorded in treatment five (planted with 20cm plant spacing). The wider the plant spacing the higher was the leaf number. However the results of the experiment did not include yield components. Thus, a similar research should be conducted so as to assure the results of this experiment and to include yield components.

Keywords: : Experiment, Garlic, Growth Performance

PROTECTIVE EFFECT OF GARLIC OIL ALONE OR COMBINED WITH LOW-DOSE GAMMA IRRADIATION ON PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS (Published)

We investigated the effect of garlic oil (GO), alone or combined with low dose total body gamma (γ)-irradiation (LDR) against paracetamol (APAP)-induced hepatotoxicity in rats. GO (100 mg/kg/day) was administered orally for 14 days before hepatotoxicity induction.Animals were irradiated 2 hours before hepatotoxicity induction by peroral APAP (1g/kg) administration, then animals were sacrificed 24 hours later.GO, alone or combined with LDR, ameliorated APAP-induced adverse effects as revealed bythe reduction ofserumliver marker enzymes activities and the histological examination. Hepatic microsomal cytochrome P2E1 activity was also reduced. Changes in hepatic redox balance were significantly attenuated by both treatments. Hepatic hydrogen peroxide was only reduced by LDR. APAP-induced mitochondrial dysfunction and apoptotic effect were also reversed. GO and/or LDR pretreatment, effectively protected against APAP-induced hepatotoxicity. Thus, the hepatoprotective effect of GO alone or combined with LDR could be of value in the protective management of APAP-induced hepatic damage

Keywords: Apoptosis, Garlic, Hepatotoxicity, Low Dose (Γ)-Radiation, Mitochondrial Dysfunction, Oxidative stress