In this study, the screening of plastic degrading microbes was done by using opaque method separately for bacteria and fungi. Four bacterial species and two fungal species which formed most opaque were used for further studies. The bacterial species was identified as Bacillus amylolyticus, Bacillus firmus, Pseudomonas putida, Pseudomonas fluroscence. Their effectiveness on the degradation of commercial polythene carry bags of Low Density Polyethylene was studied over a period of 30 days in shaker culture under laboratory conditions by weight determination method and it was found that Bacillus sp. isolated from Garbage soil degrades the plastic up to 32%. This work reveals that Bacillus amylolyticus posses greater potential to degrade plastics when compared with other bacteria.
On Some Practical Aspects of Click Thiol-Ene Reactions for Polymer Modification and Conjugation with Biomolecules (Published)
Thiol-ene reactions are among the most popular click reactions, being particularly appealing for functionalization of polymer materials and conjugation with proteins. However, although the formal definition of “click chemistry” can be very strict, one can easily arise some controversial issues related to the application of thiol-ene reactions in actual process conditions. In order to illustrate some of these issues, thiol-ene reactions were performed at conditions that simulate the conjugation of proteins with unsaturated polymers. It is shown that the presence of a heterogeneous reacting system can exert enormous impact on the performance of thiol-ene reactions, which has been largely overlooked in the literature. It is also shown that the performance of heterogeneous click reactions depends on the many constituents of the reacting system so that the design of click reactions requires detailed analysis of the desired reaction system and may not necessarily constitute a mechanistic route for immediate bioconjugation applications.
The Structural Characterizations of Polymethel(Methaacrylate) Doped Nanoparticle Metal (Cu,Co,Fe) Thin Film by Using Nd-Yag Laser Ablation (Published)
The structural properties of PMMA doped with ( Cu , Co , Fe ) nanoparticls prepared by laser ablation were studied . In this research the polymethyl mehtacrylate (PMMA) was dissolved in chloroform with (5%) concentration to form homogeneous solution. PMMA doped with (Cu ,Fe ,and Co ( nanoparticale (NPs) prepared by Nd-Yag(532nm) laser ablation. The X-ray diffraction shows that the structure of pure polymer film have an amorphous nature, but PMMA doped with (Cu , Co , Fe) nanoparticles are polycrystalline structure. The results indicate that there are a shifting and reduction in positions of peak intensity as compared with the pure (Cu , Co ,Fe) nanoparticles and there are generated some new peaks appear. The AFM examination reveal to reduction in average nanoparticle diameter after doping with NPs metal (Cu , Co , Fe ) . The FT-IR examination appear some transmittance band after doping with NPs metal (Cu , Co , Fe )
The prepared samples of thin-film by laser ablation method were achieved. Powders of doped cadmium oxide with cobalt of ratios (0,1,3,5,7)wt% were mixed and then compressing by approximately 10 tons. The samples were ablated by using Neodymium-YAG laser with 1000mW and obtained the plasma plume at 0.1mbar pressure and 250oC. Results indicate by using AFM, XRD and FTIR devices that the surface roughness and the size particle decrease with increasing dopants and there are existence stretching bonds of cadmium oxide with cobalt, and have of a multi-cubical polycrystalline .The doping leads to a small decrease in the intensity of the peaks of the cadmium oxide at (200) especially at a concentration of ratio 7%, but it was obtained lowering increase at (111), However, low peaks in most of them due to doping. Absorbance infrared ray have the greatest values of pure cadmium oxide at energies (617.61) (1117.31)(1458.62)(1624.15)(3450) cm -1 and also get a reduction in the absorption peaks as a result to doping with cobalt