Tag Archives: Fas-L

DETECTION OF SOME CYTOKINE LEVELS AND MOLECULAR ASSAY IN PSORIASIS PATIENTS (Published)

Psoriasis is a chronic, inflammatory skin disease in which recurring reddish patches, often covered with silvery scales, appear especially on the knees, elbow, scalp and trunk . In this study,one hundred and seventy -six samples were collected from psoriasis patients taken from Al-Hilla educational hospital , Marjan hospital. In order to detect IL-17 and IL-Fasl concentration in serum of psoriatic patients ,Also to determine genes for clumping factor A (ClfA) and fibronectin-binding protein A (FnbA) in Staphylococcus aureus (S.aureus )strains using PCR technique .The result show that , increased in IL-17 and IL-Fasl values in all patients group compared with healthy control, Of the total sixty-five S. aureus isolated , thirty –six of DNA sample were selected for PCR assay .Thirty –six DNA samples (100%)were CIfA gene positive ,thirty –three DNA sample (91.66%) were fnbA gene positive and three sample (8.33%) were negative for fnbA gene visualized on 1% agarose gel electrophoresis.

Keywords: Bacterialgene, Fas-L, IL-17, Psoriasis, S.aureus