Tag Archives: Escherichia Coli

Genotype, phenotype and virulence genes markers in Escherichia coli: molecular characterization and antimicrobial susceptibility associated with Diarrhoea among children in Babil province, Iraq (Published)

Background:  in Babil there are relatively few studies have been done to revealed and classify diarrheagenic Escherichia coli (DEC) strains among children with diarrhea. This study aimed at investigating DEC among children in Babil aged less than 2 years hospitalized. Methods and patients: A total of 200 children with diarrhea and 75 without diarrhea were their stools investigated using culture on Mac­Conkey and EMB agar, and the E. coli isolates were examined for detection of diarrheagenic E. coli types, antimicrobial susceptibility pattern. virulence genes detecting using PCR. A multiplex PCR system method was used to detect a species specific gene for E.coli and ten different virulence genes for detection of five pathogroups of DEC namely enteroaggregative- (EAEC), enteropathogenic- (EPEC), enterotoxigenic- (ETEC), enteroinvasive- (EIEC) and enterohemorghagic- Escherichia coli (EHEC). Results: The study has indicated that diarrheagenic E. coli isolates were found mostly in stools of children with diarrhea. DEC diarrhoeagenic E. coli were significantly detected among diarrheic children (44.8%) compared with control children (16.4%). Of the DEC pathotypes examined, EAEC was found in (64.73%), ETEC in (19.5%), EPEC in (10.5%) and EHEC in (5.27%) of diarrheic. Virulence-gene factors in DEC isolated from children with diarrhea and from controls. The distribution of virulence gene was; pCVD432 93.6%, 87.3%; eaeA 64.2%, 8.5%; bfpA 59.1%, 2.05%; stx1 22.8%, 0.8%; stx2 18.6%, 0%; estA1 heat-stable (ST) 8.14%,1.3%; estA2-4 heat-stable (ST) 14.6%, 0.2%; eltB heat-labile (LT) 43.1%, 0.4% in DEC and control children respectively

Conclusion:  This study revealed the high incidence of diarrheagenic E. coli isolates and high prevalent of antimicrobial resistance among normal intestinal E. coli with typical EAEC and typical EPEC predominating. The use of primers for both variants of ST useful to detect the sensitivity for detection of ETEC strains of hospitalized children in Babil.

Keywords: Antibiotic Susceptibility, Antimicrobial-Resistance Genes, Babil, DEC, Diarrheagenic, EAEC., EHEC, EPEC, ETEC, Escherichia Coli, Phylogenetic Grouping, Virulence Genes, diarrhea

Assessment of Bovine Raw Milk obtained from Selected Farms in Zaria Environs Nigeria for Toxigenic Strain of Escherichia coli (Published)

The presence of E. coli in food or water became accepted as indicative of recent faecal contamination and the possible presence of pathogens. The study therefore aims at detecting the presence of E.coli and E. coli O157 and an indication of quality assessment of the raw milk samples sold to the open market. A total of 199 composite milk samples and 13 bulk milk samples were collected from selected farms in Zaria environs and analyzed for total aerobic and coliform counts using standard cultural methods. The total bacterial and were more than 105 in 95.48% while total coliform counts was more than 100 cell/ml in 47.24% of the composite milk analysed. All the bulk samples collected had 100% bacterial and 69.2% coliform contamination. The cleaned teats had a mean of 3.03±2.13log10cfu/ml which was not significantly different (t=1.574, p=0.117) when compared to the count obtained from the teats that were uncleaned (3.12±1.97log10cfu/ml). However, total coliform counts of composite milk samples from animals with cleaned teats had a mean of 1.42±1.05log10cfu/ml was significantly lower (t=6.418, p-0.001) than the counts of milk obtained from cows with uncleaned teats (2.78±1.95log10cfu/ml) The incidence of Escherichia coli in the milk samples was 7.5%, when isolates were tested for enterotoxin production using the VET-RPLA kit, 14.3% of the isolates were found to possess the heat-labile toxin. All the isolates were found to be susceptible to Ceftriaxone and Ammox-Clav (n=15; %=100), one isolate was resistant to gentamycin, ciprofloxacin and chloramphenicol (n=15, 6.7%). Eight isolates were found resistant to nalixidic (n=15, 53.3%) and nine were found to be resistant to Sulphamethazole and Trimethoprim (n=15, 60%). 

Keywords: Bulk milk., Composite milk, Escherichia Coli, Toxins

Genotype, Phenotype and Virulence Genes Markers in Escherichia Coli: Molecular Characterization and Antimicrobial Susceptibility Associated With Diarrhoea among Children in Babil Province, Iraq (Published)

In Babil there are relatively few studies have been done to revealed and classify diarrheagenic Escherichia coli (DEC) strains among children with diarrhea. This study aimed at investigating DEC among children in Babil aged less than 2 years hospitalized. A total of 200 children with diarrhea and 75 without diarrhea were their stools investigated using culture on Mac¬Conkey and EMB agar, and the E. coli isolates were examined for detection of diarrheagenic E. coli types, antimicrobial susceptibility pattern. virulence genes detecting using PCR. A multiplex PCR system method was used to detect a species specific gene for E.coli and ten different virulence genes for detection of five pathogroups of DEC namely enteroaggregative- (EAEC), enteropathogenic- (EPEC), enterotoxigenic- (ETEC), enteroinvasive- (EIEC) and enterohemorghagic- Escherichia coli (EHEC). The study has indicated that diarrheagenic E. coli isolates were found mostly in stools of children with diarrhea. DEC diarrhoeagenic E. coli were significantly detected among diarrheic children (44.8%) compared with control children (16.4%). Of the DEC pathotypes examined, EAEC was found in (64.73%), ETEC in (19.5%), EPEC in (10.5%) and EHEC in (5.27%) of diarrheic. Virulence-gene factors in DEC isolated from children with diarrhea and from controls. The distribution of virulence gene was; pCVD432 93.6%, 87.3%; eaeA 64.2%, 8.5%; bfpA 59.1%, 2.05%; stx1 22.8%, 0.8%; stx2 18.6%, 0%; estA1 heat-stable (ST) 8.14%,1.3%; estA2-4 heat-stable (ST) 14.6%, 0.2%; eltB heat-labile (LT) 43.1%, 0.4% in DEC and control children respectivel. This study revealed the high incidence of diarrheagenic E. coli isolates and high prevalent of antimicrobial resistance among normal intestinal E. coli with typical EAEC and typical EPEC predominating. The use of primers for both variants of ST useful to detect the sensitivity for detection of ETEC strains of hospitalized children in Babil.

Keywords: Antibiotic Susceptibility, Antimicrobial-Resistance Genes, Babil, DEC, Diarrheagenic, EAEC., EHEC, EPEC, ETEC, Escherichia Coli, Phylogenetic Grouping, Virulence Genes, diarrhea