Vietnam is accelerating the process of industrialization and modernization of integration into the international market. The biggest strength of Vietnam in relation to other countries is the abundant human resources, able to quickly acquire technology. However, in the context of the Industrial Revolution 4.0, Vietnam’s labor productivity is still low. Therefore, it is important for commercial banks to ensure the competiveness. The study objective is to find out the determinants affecting the employees’ job motivation of Commercial Banks in Can Tho City. The researchers’ surveyed 400 employees working for commercial banks and answered 28 questions but 370 employees processed. The primary sources of data collected from November 2018 to May 2019 in Can Tho City. Simple random sampling technique. The Data analyzed Cronbach’s Alpha and the exploratory factor analysis (EFA), which used for multiple linear regression and using partial least squares method. Employees’ responses measured through an adapted questionnaire on a 5-point Likert scale. In addition, the findings of the study have six factors affecting the employees’ job motivation of Commercial Banks in Can Tho City with significance level 0.05.