Human resource is considered the most valuable asset of a bank and different factors affect its performance and efficiency. In today’s world of non-stop change, in which banks are competing with all their capabilities, a big portion of bank’s energy is put into the attention given to staff. The purpose of this study was to examine the impact of the factors affecting the service motivation of the staffs of commercial banks. This is a quantitative research study and data were collected through questionnaire containing a 5-point Likert scale. The findings of this study revealed that five factors enhance the motivation whereas administrative policies improve the motivation of bank staffs. The study results showed that there were 300 staffs related to bank sector in Ho Chi Minh City who interviewed and answered about 22 questions. The Data collected from June 2016 to July 2017. This study had been analyzed Cronbach’s Alpha testing, KMO testing and the result of KMO testing used for the multiple regression. Persons’ responses measured through an adapted questionnaire for staffs. The Data processed by SPSS 20.0. In addition, five components affecting staffs’ service motivation of commercial banks in Ho Chi Minh City with significance level 5 percent and then the researcher has recommendations improving the staffs’ service motivation in the future.