EXPRESSION OF VEGF AND BMP7 IN BONE HEALING AFTER TOPICAL, SYSTEMIC FLUORIDE APPLICATION (EXPERIMENTAL STUDY IN RATS) (Published)
Bone repair is a multistep process involving migration, proliferation, differentiation and activation of several cell types. The effect of topical and systemic application of fluoride on bone healing was studied by histological and immunohistochemical evaluation for expression of VEGF and BMP7. Seventy two Swiss rats were used in the present study. Bone defect was created in rat femur with topical application of fluoridated tooth paste and systemic fluoride through water supplement, while control group left without any application. The animals were sacrificed at periods 3, 7, 14, and 28 days. Six rats for each group and for each period. Histological and immunohistochemical evaluation for VEGF and BMP7 were carried out for all animals.The Histological findings illustrated that all study groups showed deposition of osteoid tissue at 7 days and mature bone illustrated only in paste group at 28 days. The Immunohistochemical findings of VEGF for positive expression by bone marrow stromal cells and bone tissue cells reveled statistically to be the highest in paste group and at 14 days period. While positive expression of BMP7 illustrated positivity in different percentage and the paste group recorded the highest value at 3 days period. It was concluded that fluoridated tooth paste can affect more in bone maturation and had effect on VEGF and BMP7 expression.
Keywords: Bone Healing., Bone Morphogenic Proteins, Fluoride Tooth Paste, Vascular Endothelial Growth Factor