Bone repair is a multistep process involving migration, proliferation, differentiation and activation of several cell types that leads to healing . Many biological activities associated with Aloe vera have been attributed to the chemical component contained in the gel of the leaves. The present study was designed to illustrate the effect of topical application of aloe vera on bone healing by histological examination and immunohistochemical evaluation of BMP7 .Fifteen male Swiss rats were subjected for femur bone defect , one in the left side represented the control(defect was left without treatment) ,while the right side represented the experimental(treated with 0.1 µl of aloe vera gel). The animals were sacrificed at periods 7, 14, and 28 day, as five rats for each period. Histological and immunohistochemical evaluation for expression of BMP7 were done for all study samples.In-vivo results showed aloe vera treated group had faster bone healing compared with untreated controls. A substantial findings for the growth of bone trabeculae was observed in treated group. The results revealed that A.vera significantly increased osteogenic cell proliferation, expression of bone morphogenetic protein7and maturation of newly formed bone,compared with the untreated group. Conclusion:These data suggest aloe vera could function as a bioactive molecule inducing bone formation by stimulating proliferation and differentiation of osteoblasts with high expression of BMP7.
EXPRESSION OF VEGF AND BMP7 IN BONE HEALING AFTER TOPICAL, SYSTEMIC FLUORIDE APPLICATION (EXPERIMENTAL STUDY IN RATS) (Published)
Bone repair is a multistep process involving migration, proliferation, differentiation and activation of several cell types. The effect of topical and systemic application of fluoride on bone healing was studied by histological and immunohistochemical evaluation for expression of VEGF and BMP7. Seventy two Swiss rats were used in the present study. Bone defect was created in rat femur with topical application of fluoridated tooth paste and systemic fluoride through water supplement, while control group left without any application. The animals were sacrificed at periods 3, 7, 14, and 28 days. Six rats for each group and for each period. Histological and immunohistochemical evaluation for VEGF and BMP7 were carried out for all animals.The Histological findings illustrated that all study groups showed deposition of osteoid tissue at 7 days and mature bone illustrated only in paste group at 28 days. The Immunohistochemical findings of VEGF for positive expression by bone marrow stromal cells and bone tissue cells reveled statistically to be the highest in paste group and at 14 days period. While positive expression of BMP7 illustrated positivity in different percentage and the paste group recorded the highest value at 3 days period. It was concluded that fluoridated tooth paste can affect more in bone maturation and had effect on VEGF and BMP7 expression.