Bio-Priming to Improve the Seed Germination, Emergence and Seedling Growth of Kale, Carrot and Onions (Published)
There is increasing interest in the use of bio-priming as a means of growth promotion through the efficient use of Plant Growth Promoting Rhizobacteria as an economic and efficient means of treating crops. This study was carried out to examine the effect of bio -priming using Bacillus subtilis and Serratia nematodiphila on the seed germination, seed emergence and plant growth of the seedlings of vegetable seeds (kale var. Collard, carrots var. Nantes, and onion var. Detroit red). Bio-priming involved soaking 2 g of seeds in 5ml/l for both Bacillus subtilis ( 2.4×107 cfu/ml) and Serratia nematodiphila solution (2.4 cfu/ml) and distilled water (control) for 20 minutes and then left to air dry for 30 minutes. Germination was assessed after 8 days. Seedling emergence and growth was assessed by sowing treated seeds in a seedling media in a controlled environment. The results show that combined germination percentage of all three species for the three treatment was: Water control 59.00%, Bacillus 65.33% and Serratia nematodiphila 69.66%, water being significantly lower (P= 0.05) than the two microbial treatments. The combined mean height of all three species for the three treatments after three weeks was: Water control 4.33 cm, Bacillus subtilis 8.68cm and Serratia nematodiphila 9.31cm, water being significantly lower (P= 0.05) than the two microbial treatments.
Varietal Resistance of Sunflower and Okra Bio-Primed Seeds Against Root Infecting Fungi and Establishment of Crop Plants (Published)
Okra (Abelmoschus esculentus L.) varieties like OH-152, Arka anamika and unknown variety and sunflower (Helianthus annuus L.) varieties like S-278, Hysun-39 and unknown variety after bio-priming with leaf extracts of Acacia nilotica (L.) Willd. ex Delile Sapindus mukorossi (L.) and microbial antagonists (Trichoderma harzianum and Rhizobium meliloti) at different time intervals (10, 20 minutes) were screened against root infecting fungal pathogens (Macrophomina phaseolina, Rhizoctonia solani and Fusarium spp) and growth of crop plants. Results obtained showed that among all the three varieties of sunflower, variety S-278 after bio-priming with A. nilotica leaf extract for 10 minutes and T. harzianum conidial suspension for 20 minutes was found to be most effective for the establishment of plants and completely control the colonization of M. phaseolina followed by Hysun-39 and unknown variety. Whereas in case of okra, variety OH-152 after bio-priming with A. nilotica leaf extract, T. harzianum and R. meliloti cell/conidal suspension for 10 minutes was recorded to be most effective for the complete inhibition of M. phaseolina and significant elevation of growth of plants followed by A. anamika and unknown varieties