LACTOBACILLUS SALIVARIUS BACTERIOCIN AND SUPERNATANT ACTIVITY AGAINST ENTAMOEBA HISTOLYTICA IN VITRO AND IN VIVO (Published)
Lactobacillus salivarius is a probiotic bacteria species found in the gastrointestinal tract and breaks and exert a range of therapeutic properties, so antiamoebic activity of Lactobacillus salivarius supernatant and bacteriocin were tested in vitro and in vivo on Entamoeba histolytica and using metronidazole as a reference amoebicidal agent. In vivo supernatant have a clear impact more than bacteriocin and metronidazole where led to the disappearance of parasite in the feces of infected mice after 9th day while bacteriocin and metronidazole were the 12th, 11th day respectively, the disappearance of the parasite after dosing while the results were expected to reverse invitro as it was observed that the effect was more of bacteriocin it leads to lower growth rate to (35 – 30.25×104 cell ∕ ml) after 24 and 48hr while the supernatant and metronidazole were lower growth rate after 24 and 48hr (50 – 42×104 cell ∕ ml), (44.5 – 40×104 cell ∕ ml) respectively, compared to control group whose results were (55.5 – 60.75×104 cell ∕ ml). Histological changes also explained that bacteriocin effects were clearly on intestinal tissue while the supernatant was safly and harmless.
Keywords: Entamoeba histolytica, Lactobacillus, Lactobacillus salivarius, bacteriocin, supernatant.