Tag Archives: Bacillus sp.

Characterization and Application of Biopolymer Producing Bacteria for Enhanced Oil Recovery (Published)

The objective of this research is to isolate and identify hydrocarbon-degrading bacteria for biopolymer synthesis and application in the augmentation of Nigerian heavy crude oil recovery. MEOR refers to the process of injecting either indigenous or non-indigenous microbes into hydrocarbon reserves. Injecting microorganisms with nutritional broth facilitate the formation of essential metabolites such as biosurfactants, biopolymers, and gases, resulting in decreased interfacial tension, viscosity modification, and mobility control. It is environmentally friendly, less expensive to implement, and requires minimal or no changes to the existing infrastructure. A soil sample from a hydrocarbon-contaminated site in Ogoniland was collected and sent to a laboratory for physicochemical and microbiological investigation. Bacillus sp, Pseudomonas sp, and Klebsiella sp were biochemically identified after screening three isolates for biopolymer production using Sudan black solution. To assess the ideal growth and biopolymer synthesis capability under reservoir conditions, a variety of pH, temperature, salinity, carbon, and nitrogen nutrition sources were applied to selected microorganisms. Peptone is the optimal nitrogen source for Bacillus sp, glucose is the optimal carbon source for Bacillus sp, and glycerol is the optimal carbon source for Pseudomonas sp and Klebsiella sp, as indicated by the results. In addition, the following are the ideal parameter ranges for the three microorganisms: pH 7–8, a temperature range between 25 and 350 degrees Celsius, and a salinity range between 0.5 and 5% are all desirable conditions for a body of water. After inoculation with microorganisms and the optimum nutrient source, an additional recovery range of 18.33% to 29.09% of the pore capacity was achieved. The post-recovery analysis uncovered a remarkable transformation of heavy crude to light hydrocarbon components by an average of 20.33 percent with glucose and 97.27 percent with peptone.

Keywords: Bacillus sp., Microorganisms, Pseudomonas sp and Klebsiella sp, biochemical characterization, biosurfactant, physicochemical and microbiological analyses., tertiary recovery


Objective of the research was to create bio-activator as decomposer in empty recemus of oil palm. The research was conducted for two years, however, in the first year, two types of bio-activators were made of different materials using fruit’s wastes and banana’s capitulum. Then, the organic fertilizers were made of empty recemus of oil palm by the assistance of bio-activator. In the second year, the organic fertilizers were applied on the cropland of soybean plots. Such bio-activator was taken the banana plant, in which the fruits had been taken (harvested), while the fruit’s wastes were obtained from the fruit’s sellers. The bio-activator was made at the Laboratory of Agronomy, State Polytechnics of Agriculture, Samarinda. Such bio-activator was made through fermentation. A ton of empty recemus was taken from waste of the oil palm’s mill. Such empty recemus were chopped and given the bio-activator evenly, and then fermented until they are ready to be used. Results of the research on tankos compost from MOL of fruits showed low nutrients level, such as : N 0.25 %, P2O5 0.19 % , K2O 0.82 %, MgO 0.22 %, Pb 188.74 %, Ca 6. 29 %, Cu 33.56 %, Zn 57.40 %, Fe 1744.60 % , Mn 72.42 % and result of tankos compost from MOL of banana’s capitulum have still showed relatively low nutrients level, such as : N 1.25 %, P2O5 0.27 %, K20 0.94 % , MgO 0.23 %, Pb 425,66 %, Ca 6.95%, Cu 27.52 % , Zn 59.49 % , Fe 3593.20 % , Mn 77.95%. Results of the bacterial identification on MOL of fruits and MOL of banana’s capitulum showed one type of bacteria for fruit MOL, such as Bacillus sp., and the banana’s capitulum MOL, such as Enterobacter sp.

Keywords: Bacillus sp., Empty Recemus of Oil Palm, Enterobacter sp