Tag Archives: Antibody

Seroprevalence of Rubella IGG Antibodies among Pregnant Women Attending Antenatal Clinics in Abakaliki, Ebonyi State, Nigeria (Published)

The Seroprevalence of rubella IgG antibodies was investigated among 187 pregnant women attending Mile Four Hospital (MFH) Abakaliki, Ebonyi State. Blood specimens were collected from the subjects and sera extracted. The sera were screened for rubella virus immunoglobulin G antibody (IgG) using ELISA test Kit (Micro Immune limited, UK). The results obtained were subjected to SPSS system and Chi square test for significant difference at 95% confidence interval. Of the 187 pregnant women tested, 174(93.05 %) were positive for the rubella virus IgG. This study revealed that all the subjects who were 41 years and above tested positive 5(100.00%), whereas those who were 15 years and below 2 subjects had the prevalence rate of 50.00%. Pregnant women in their third, first and second trimesters had prevalence rates of 95.75%, (95.35%) and 86.95% accordingly. Samples tested shows that unmarried women were more infected with anti- rubella IgG of 94.44% recorded, then followed by married women with 93.79% and divorcee  had 90.91%. However, widows had the least prevalence rate of 84.62%. Occupationally, women civil servants had prevalence rate of 97.56%. Educationally, pregnant women with tertiary education were more infected, rubella virus amounting to 96.61%. More infection was found among women living in rural areas with 93.59% rate, than their urban counterparts who had 92.66%. Furthermore, primigravida women were more infected (95.16%). The Sero-prevalence of rubella in this study was high and it calls for general surveillance and mass immunization of children and females of child bearing age.

Keywords: Antibody, ELISA, Immunnoglobulin G, Pregnant Women, Rubella, Seroprevalence.

Mechanisms of Immunity to Escherichia Coli O157:H7 In Albino Rats: Role Of Homologous Antibodies (Published)

The role of antibodies in conferring protection against infection caused by Escherichia coli O157:H7 in albino rats was carried out in this study. Antibody against E. coli O157:H7 of known titre was raised actively and used to passively protect some sets of rats intraperitoneally. Antibody titre was determined using tube agglutination test. At different intervals, starting from 30 minutes to 168 hours, the liver, spleen and ileum of actively, passively immunized and unimmunized rats were dissected out and the number of trapped E. coli O157:H7 was counted in these selected organs using standard microbiological techniques. The highest recovery of the organism was seen in unimmunized rats followed by the actively immunized rats. The least recovery was seen in the passively immunized rats. The spleen trapped more of the organism compared to the liver and ileum throughout the experiment. From this investigation therefore, passively raised antibodies to E. coli O157:H7 play a significant role by causing a reduction in the number of trapped E. coli O157:H7 in the organs examined. It is conceivable therefore that in case of an outbreak of infection caused by this organism, administration of preformed antibodies against E. coli O157:H7 to infected individuals, that is, passive immunity will reduce the severity of the infection and also the spread of the infection in the community.

Keywords: Antibody, E. coli O157:H7, Ileum, Immunization, Liver, Spleen

Mechanisms of Immunity to Escherichia Coli O157:H7 In Albino Rats: Role Of Homologous Antibodies (Published)

The role of antibodies in conferring protection against infection caused by Escherichia coli O157:H7 in albino rats was carried out in this study. Antibody against E. coli O157:H7 of known titre was raised actively and used to passively protect some sets of rats intraperitoneally. Antibody titre was determined using tube agglutination test. At different intervals, starting from 30 minutes to 168 hours, the liver, spleen and ileum of actively, passively immunized and unimmunized rats were dissected out and the number of trapped E. coli O157:H7 was counted in these selected organs using standard microbiological techniques. The highest recovery of the organism was seen in unimmunized rats followed by the actively immunized rats. The least recovery was seen in the passively immunized rats. The spleen trapped more of the organism compared to the liver and ileum throughout the experiment. From this investigation therefore, passively raised antibodies to E. coli O157:H7 play a significant role by causing a reduction in the number of trapped E. coli O157:H7 in the organs examined. It is conceivable therefore that in case of an outbreak of infection caused by this organism, administration of preformed antibodies against E. coli O157:H7 to infected individuals, that is, passive immunity will reduce the severity of the infection and also the spread of the infection in the community.

Keywords: Antibody, E. coli O157:H7, Ileum, Immunization, Liver, Spleen