Tag Archives: Antibiotic resistance

Microbial Composition, Antibiotic Sensitivity and Proximate Composition of Popular Imported Powdered Infant Milk Formulas sold in Ado Ekiti, Nigeria. (Published)

The study was carried out to determine the microbial and proximate composition of  popular imported infant milk formulas in retail market at Ado-Ekiti. A total of six popular commercial infant milk poducts were purchased from the main market in Ado- Ekiti, Nigeria, and evaluated for microbial composition: total bacterial, coliform and fungal counts. Their proximate components were also determined. The total bacterial count ranged from 0.6×103 to 0.9×104 CFU/ml, with mean value of 3.4×103 CFU/g, the total coliform ranged from 0.2×103 to 0.6×104 CFU/ml, with the mean value of 1.7 x 103 CFU/g and the total fungal count ranged from 0.2×103 to 0.5×104 CFU/ml, with the mean value of 0.9 x103 CFU/g. Enteric bacteria and mycotoxigenic fungi were isolated from the formulas. Having subjected all the bacteria isolates to antibiotic susceptibility test, they all showed multiple antibiotic resistance index. The fat content ranged from 10mg/100g to 27.7mg/100g with mean value of 17.8mg/100mg;  protein content, 1.5mg/100mg  with mean value of 10.68mg/100mg and carbohydrate content ranged from 7.2mg/100mg with the mean value of 50.76 mg/100g. However  ash, moisture and fibre contents of all the samples were insignificant. This study depicted the possibility of microbial contamination in infant milk formula supposedly sterile.

Keywords: Antibiotic resistance, Infant milk formula, Microbial count, Proximate

Study on Enumeration, Characterization and Antibiotic Susceptibility of Coagulase positive Staphylococci Isolated from Hand Pump Water (Published)

A study was conducted on bacteriological analysis of hand pump water for determining the quality and extent of contamination. A total of 100 hand pump water samples were studied for enumeration of typical staphylococci (TS) and Coagulase positive staphylococci (CPS). On m-staphylococcal agar 82% samples yielded typical staphylococcal colonies, 79% of which was in the range of 1-20 cfu/100ml, 15% in the range of 20-40 and 2% were between 100-500 staphylococci/100ml. Coagulase positive staphylococcus were found in 25 samples, of which 76% had a range of 1-20 and remaining 24% had count less than 100 cfu/100ml of hand pump water tested. Minimum value was 1 and maximum 364 for TS while for CPS minimum value was 2 and maximum was 86 cfu/100ml, with a mean value of 80.5 and 15.86 respectively. From 82 samples, 106 colonies randomly selected among 1733 golden to orange colonies of TS obtained on m-staphylococcal agar, were subjected to coagulase positive test.  Thirty two coagulase positive strains from 25 positive samples were isolated and characterized.  Twenty five were Mannitol fermentation positive while seven were negative. In our study 30% typical staphylococci were found pathogenic i.e. coagulase positive.  A varying degree of resistance and susceptibility was found against twelve antibiotics. Ampiclox was found 100% effective for these CPS strains.

 

Keywords: Antibiotic resistance, Coagulase positive Staphylococci, Ground water, Staphylococci

Microbial Composition, Antibiotic Sensitivity and Proximate Composition of Popular Imported Powdered Infant Milk Formulas sold in Ado Ekiti, Nigeria (Published)

ABSTRACT: The study was carried out to determine the microbial and proximate composition of popular imported infant milk formulas in retail market at Ado-Ekiti. A total of six popular commercial infant milk poducts were purchased from the main market in Ado-Ekiti, Nigeria, and evaluated for microbial composition: total bacterial, coliform and fungal counts. Their proximate components were also determined. The total bacterial count ranged from 0.6×103 to 0.9×104 CFU/ml, with mean value of 3.4×103CFU/g, the total coliform ranged from 0.2×103 to 0.6×104 CFU/ml, with the mean value of 1.7 x 103CFU/g and the total fungal count ranged from 0.2×103 to 0.5×104 CFU/ml, with the mean value of 0.9 x103CFU/g. Enteric bacteria and my cotoxigenic fungi were isolated from the formulas. Having subjected all the bacteria isolates to antibiotic susceptibility test, they all showed multiple antibiotic resistance index. The fat content ranged from 10mg/100g to 27.7mg/100g with mean value of 17.8mg/100mg; protein content, 1.5mg/100mg with mean value of 10.68mg/100mg and carbohydrate content ranged from 7.2mg/100mg with the mean value of 50.76 mg/100g. However ash, moisture and fibre contents of all the samples were insignificant.

This study depicted the possibility of microbial contamination in infant milk formula supposedly sterile.

 

Keywords: Antibiotic resistance, Infant milk formula, Microbial count, Proximate

Multi-Drug Resistant Staphylococcus aureus from Poultry Farms in Ebonyi State, Nigeria. (Published)

Over the years, methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a nosocomial and community pathogen worldwide, causing a plethora of diseases. A distinctive type of MRSA has also emerged in livestock and companion animals. Its isolation in chicken has been reported in some countries and its propensity for zoonotic transmission potentially represents a serious risk-factor for poultry farm workers and the general population. Nasal and cloacae swabs of chickens selected at random from 9 poultry farms and clinical isolates of staphylococci from Ebonyi State, Nigeria, were collected and screened for S. aureus using standard microbiological procedures. Antibiotic susceptibility pattern of the S. aureus to a panel of 14 commonly used and regulated antibiotics in the area were determined using the Kirby-Bauer disc agar diffusion (DAD) method according to the Clinical Laboratory Standards Institute (CLSI). Out of a total of 325 S. aureus isolated, 76% (247/325) were from poultry, while 24% (78/325) were from the clinics. The prevalence rates of S. aureus in broilers and layers were 49% and 51% respectively. The percentage carriage of MRSA in poultry was 6.1% and 15.3% in the clinics. The percentage of isolates showing multi antibiotic resistance index (MARI) of 0.3 and above was 13.97% displaying 46 antibiotic resistance patterns. All the methicillin-resistant S. aureus (MRSA) were multidrug resistant. This underscores the need for discretion in the application of antibiotics in animal feeds and its empirical use in the hospitals.

Keywords: Antibiotic resistance, Isolates., MRSA, Multidrug-Resistance-Index, Poultry, Staphylococcus Aureus

Microbial Composition, Antibiotic Sensitivity And Proximate Composition Of Popular Imported Powdered Infant Milk Formulas Sold In Ado Ekiti, Nigeria (Published)

The study was carried out to determine the microbial and proximate composition ofpopular imported infant milk formulas in retail market at Ado-Ekiti. A total of six popularcommercial infant milkpoducts were purchased from the main market in Ado-Ekiti, Nigeria, and evaluated for microbial composition: total bacterial, coliform and fungal counts. Their proximate components were also determined. The total bacterial count ranged from 0.6×103 to 0.9×104 CFU/ml, with mean value of 3.4×103CFU/g, the total coliform ranged from 0.2×103 to 0.6×104 CFU/ml, with the mean value of 1.7 x 103CFU/g and the total fungal countranged from 0.2×103 to 0.5×104 CFU/ml, with the mean value of 0.9 x103CFU/g. Enteric bacteria and mycotoxigenic fungi were isolated from the formulas. Having subjected all the bacteria isolates to antibiotic susceptibility test, they all showed multiple antibiotic resistanceindex. The fat content ranged from 10mg/100g to 27.7mg/100g with mean value of 17.8mg/100mg; protein content, 1.5mg/100mg with mean value of 10.68mg/100mg and carbohydrate content ranged from 7.2mg/100mg with the mean value of 50.76 mg/100g. However ash, moisture and fibre contents of all the samples were insignificant.This study depicted the possibility of microbial contamination in infant milk formula supposedly sterile.

Keywords: Antibiotic resistance, Infant milk formula, Microbial count, Proximate

MICROBIAL COMPOSITION, ANTIBIOTIC SENSITIVITY AND PROXIMATE COMPOSITION OF POPULAR IMPORTED POWDERED INFANT MILK FORMULAS SOLD IN ADO EKITI, NIGERIA (Published)

The study was carried out to determine the microbial and proximate composition ofpopular imported infant milk formulas in retail market at Ado-Ekiti. A total of six popularcommercial infant milkpoducts were purchased from the main market in Ado-Ekiti, Nigeria, and evaluated for microbial composition: total bacterial, coliform and fungal counts. Their proximate components were also determined. The total bacterial count ranged from 0.6×103 to 0.9×104 CFU/ml, with mean value of 3.4x103CFU/g, the total coliform ranged from 0.2×103 to 0.6×104 CFU/ml, with the mean value of 1.7 x 103CFU/g and the total fungal countranged from 0.2×103 to 0.5×104 CFU/ml, with the mean value of 0.9 x103CFU/g. Enteric bacteria and mycotoxigenic fungi were isolated from the formulas. Having subjected all the bacteria isolates to antibiotic susceptibility test, they all showed multiple antibiotic resistanceindex. The fat content ranged from 10mg/100g to 27.7mg/100g with mean value of 17.8mg/100mg; protein content, 1.5mg/100mg with mean value of 10.68mg/100mg and carbohydrate content ranged from 7.2mg/100mg with the mean value of 50.76 mg/100g. However ash, moisture and fibre contents of all the samples were insignificant.This study depicted the possibility of microbial contamination in infant milk formula supposedly sterile.

Keywords: Antibiotic resistance, Infant milk formula, Microbial count, Proximate