Background: This descriptive cross sectional and analytical study has been done to study prevalence of Human immunodeficiency virus (HIV) infection and the impact of associated factors affecting prevalence in Ethiopian people in west Juref in Khartoum state. Methods: Seventy five blood samples collected from patients coming to Alfaruk medical center, seeking for medical care. Serum was separated from blood and subjected to rapid testing by immunochromatogrphy (ICT) to detect presence of antibody against HIV antigen. Result: Six samples were positive for presence of HIV-Abs (5-positive for HIV-1 and 1 positive for HIV-2) comprising 8% while (69) samples (92%) were negative for HIV.ELISA (Fourth generation ELISA) was done on the six positive ICT result as confirmatory test revealed presence of strong positive result indicating presence of HIV-Ab. Conclusion: Results of studying parameters of socioeconomic factors affecting. HIV prevalence indicates positive correlation showing high infection rateIn the age group (31-40years), in males (8%) more than females, all males were Christian, and maybe uncircumcised, in the same time uneducated or just at primary level.