Characterization and Antimicrobial Properties of Volatile Component of the Ethanol Leaf Extract of Momordica Charantia

Abstract

GC-MS characterization of the leaf extract of Momordica charantia  was carried out with SHIMAZU Japan Gas Chromatography 5890-11 with a fused GC column OV 101 coated with polymethyl silicon (0.25 mm x 50 m) The initial phytochemical screening of the sample  revealed the  presence of alkaloids, saponins, flavonoids, phenols, steroids glycosides and tannins .the interpretation of the GC/MS spectrum revealed 13 absorption peaks Peak 1 was identified as Benzene  propanoic acid,with molecular weight of 150g and a molecular formula of C9H10O2.   Similarly, peak 2-13 were also identified as 1- Octadecyne, 1-Tridecyne,  Pentadecanoic acid methyl ester,  Hexadecanoic acid ethyl ester, n-Hexadecanoic, 11-Octadecanoic acid methyl ester, Phytol ,Oleic acid, 1-fluoro decane, E-9-Tetradecenal, 9-Octadecenal, 1-Pentanol-4-methyl-2-propyl with the following molecular weights; 250g,180g,270g,284g,256g,296g,296g,282g,160g,  210g, 266g,144g and molecular formula of C18H34.,  C13H24,  C17H34O2, C18H36O2.  C16H32O2, , C19H36O2 , C20H40O.  C18H34O2.  C10H21F. C14H26O. C18H34O.  C9H20O respectively. The leaf extract showed marked inhibition of some of the selected pathogens,. At concentrations of 200mg/cm3 , the extract  inhibited Staphylococcus aureus, 4mm, Trichophyton 2mm, Candida albican 4mm and streptococcus auerus 10mm  The minimum inhibition concentrations are 200mg/cm3 for trichophyton spp, 20mg/cm3 for Candida albicans, 100mg/cm3 for staphylococcus aureus and 50mg/cm3 for streptococcus aureus .

Keywords: Antimicrobial properties, Ethanol Leaf, Momordica Charantia, Volatile Component


Article Review Status: Published

Pages: 14-27 (Download PDF)

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