The purpose of this study was to find out gender differences in performance in mathematics among Pre-service Teachers in the Brong-Ahafo Region of Ghana. A sample of one hundred pre-service teachers drawn by convenience sampling from the public College of Education was used for the study. The one hundred pre-service teachers which consist of fifty males and fifty females were conveniently selected from the second year form. The design for the study was a descriptive survey and for their performance in mathematics, pre-service teachers’ actual examination scores were collected from the public College of Education and were analysed using the t-test in SPSS. The test was done on performance of pre-service teachers. Based on the findings of this study, there was a significant difference in their performance. Finally, it was also recommended that administrators and mathematics tutors in the public College of Education in the Brong-Ahafo Region should adopt strategies that suggest to pre-service teachers that they have what it takes to go through their studies successfully, and should focus their attention on how to reduce skills disparities among pre-service teachers.
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