Genotype, phenotype and virulence genes markers in Escherichia coli: molecular characterization and antimicrobial susceptibility associated with Diarrhoea among children in Babil province, Iraq

Abstract

Background:  in Babil there are relatively few studies have been done to revealed and classify diarrheagenic Escherichia coli (DEC) strains among children with diarrhea. This study aimed at investigating DEC among children in Babil aged less than 2 years hospitalized. Methods and patients: A total of 200 children with diarrhea and 75 without diarrhea were their stools investigated using culture on Mac­Conkey and EMB agar, and the E. coli isolates were examined for detection of diarrheagenic E. coli types, antimicrobial susceptibility pattern. virulence genes detecting using PCR. A multiplex PCR system method was used to detect a species specific gene for E.coli and ten different virulence genes for detection of five pathogroups of DEC namely enteroaggregative- (EAEC), enteropathogenic- (EPEC), enterotoxigenic- (ETEC), enteroinvasive- (EIEC) and enterohemorghagic- Escherichia coli (EHEC). Results: The study has indicated that diarrheagenic E. coli isolates were found mostly in stools of children with diarrhea. DEC diarrhoeagenic E. coli were significantly detected among diarrheic children (44.8%) compared with control children (16.4%). Of the DEC pathotypes examined, EAEC was found in (64.73%), ETEC in (19.5%), EPEC in (10.5%) and EHEC in (5.27%) of diarrheic. Virulence-gene factors in DEC isolated from children with diarrhea and from controls. The distribution of virulence gene was; pCVD432 93.6%, 87.3%; eaeA 64.2%, 8.5%; bfpA 59.1%, 2.05%; stx1 22.8%, 0.8%; stx2 18.6%, 0%; estA1 heat-stable (ST) 8.14%,1.3%; estA2-4 heat-stable (ST) 14.6%, 0.2%; eltB heat-labile (LT) 43.1%, 0.4% in DEC and control children respectively

Conclusion:  This study revealed the high incidence of diarrheagenic E. coli isolates and high prevalent of antimicrobial resistance among normal intestinal E. coli with typical EAEC and typical EPEC predominating. The use of primers for both variants of ST useful to detect the sensitivity for detection of ETEC strains of hospitalized children in Babil.

Keywords: Antibiotic Susceptibility, Antimicrobial-Resistance Genes, Babil, DEC, Diarrheagenic, EAEC., EHEC, EPEC, ETEC, Escherichia Coli, Phylogenetic Grouping, Virulence Genes, diarrhea


Article Review Status: Published

Pages: 11-20 (Download PDF)

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