Effect of Chemical, Organic and Biological Fertilizers on Protein Concentration and Protein Electrophoretic Profiles of Wheat Plants Irrigated with Seawater

Abstract

Wheat plants grown under 0%, 20% and 40% of seawater, 0, 100, 250 and 500 kg/ha of urea as chemical fertilizer; Rhizobium and Azotobacter as biofertilizer; and 0, 5, 10 and 20 kg/ha of humic acid as organic fertilizer. Soluble, insoluble and total proteins as well as RAPD-PCR were evaluated. To obtain reliable molecular markers for response to salinity in such genotype, RAPD banding patterns by using two primers. It was found that low concentration (20%) of seawater caused an observed increase in soluble protein. While, high concentration (40%) of seawater caused a significant decrease in soluble and insoluble proteins as well as total proteins. Biological and organic fertilizer treatments increased total proteins even at 40% seawater treatment as compared with unfertilized plants at the same level of seawater. The results of RAPD analysis showed that the two primers (OPUPC-75 and OPA18 could efficiently align genomic DNA of wheat. Approximately 88 bands (AF) were amplified under different treatments using the two primers. Monomorphic and polymorphic bands ware present in all individuals, and the mean percentage of polymorphic bands for all treatments was 76.2%, with molecular sizes ranging from 350 to 1900 pb. It was observed also that eight bands of the 88 commonly detected in all the samples, so it could be the specific genus bands of Triticum aestivum species. It seems that the extensive polymorphism detected among seawater and fertilizertreatments elevated the degree of change occurring in DNA sequences

Keywords: Salinity, Sorghum, chlorophyll., electrophoretic patterns, soluble proteins


Article Review Status: Published

Pages: 29-46 (Download PDF)

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