The 15 Actinobacteria strains isolated from the Daqu for light-flavour Chinese liquor, were identified on the basis of 16S rDNA gene sequence analysis and investigated from morphological, physiological and biochemical characteristics to analyze the role in Fenjiu liquor brewing. The research results demonstrated the 10 strains have the ability to produce exo-enzymes, amylase, protease, cellulose simultaneously. The 15 strains showed a wide spectrum of antifungal and antibacteria properties on tests of antagonisma against phytopathogenic fungi and bacteria. The 16S rDNA sequencing analysis revealed that Streptomyceswas is the predominant genus in culture-dependent Actinobacteria strains in Daqu ecosystem. We speculated the Actinobacteria play a very important role on two aspects: one is the ability to produce amylase, protease, cellulase to participate in the process of saccharification; the other is the inhibition of harmful microorganisms in the production of Daqu, and these isolates may have strong biotechnological potential in agriculture.
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