Studies on nutritional composition and some anti-nutritional factors of three edible mushroom species in South Eastern Nigeria such as Termitomyces sp, Russula sp and Pleutotus tuber regium was carried out. Standard analytical methods were used to obtain the proximate composition, mineral composition, vitamin C content anti-nutritional factors (phytin, hydrocyanide, and tannin). The moisture content was highest in the Russula sp (90.2%) and lowest in the Pleutorus tuber regium (60.7%). On the average the crude protein was 41.7%, 30.3% and 15.4% on dry mass basis (dmb) for Termitomyces sp, Russula sp and Pleutorus tuber regium respectively, while the values for crude fibre were 5%, 17.9% and 13.5 respectively. Carbohydrate content ranged from30.2 to 55% while fat content ranged from 3.6% to 7.8% (dmb). Mineral composition indicated appreciable amounts of iron 2093.3 ppm in T. robustus and Rusular 1830.0 ppm and 2001.6 ppm respectively. Other mineral elements such as potasium and phosphorus were also appreciable. The least in all the species examined was Calcium. Vitamin C content was very low (0.01- 0.04 %) in all the species. Phytic acid content ranged from 0.94 – 0.75mg/100g, Hydrocyanic acid from 0.04 – 1.0mg/100g, and tannin was from 0.04 to 0.13 which is quite low to give adverse effect.
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