Plant Regeneration of A. Lakoocha from Encapsulated Nodal Explants

Abstract

One of the alternative methods adopted in recent years is to use biotechnological approaches for improving the tree species. A proficient protocol for encapsulation of nodal segments of Artocarpus lakoocha Roxb has been developed for plant regeneration through non-embryogenic synthetic seeds. Concentrations of sodium alginate and calcium chloride greatly affected morphology and texture encapsulating gel. 3 % sodium alginate with 100 mM CaCl2 has been found to be best possible concentration for the production of identical synthetic seeds.  25 days old in vivo plantlet of A. lakoocha was used for obtaining nodal segments, used in all experiments.  Five experiments were performed using MS medium (agar solidified and liquid) supplemented with 1, 3 and 5 mg/l BAP. Concentration of 1 mg/l BAP was observed to be more effective for achieving highest regeneration frequency in comparison to other concentration of BAP (3 and 5 mg/l). In third experiment only different strength of MS medium was used , in last two experiments nodal segments prior encapsulation and encapsulated nodal segments were given pulse treatment  with (IBA)for 48 hours and  concentration of 3 mg/l BAP was observed to be most effective for  synthetic seed regeneration. Data were recorded after 4 weeks of culture.

Keywords: Acclimatization, BAP, IBA, MS medium, Pulse treatment, synthetic seeds


Article Review Status: Published

Pages: 8-17 (Download PDF)

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