This study was an attempt to examine expression of LasR and LasI genes in quorum sensing system under the influence of iron oxide nanoparticles modified with silver surface structure in the clinical strain of Pseudomonas aeruginosa. The participants of this study 51 samples of Pseudomonas aeruginosa bacteria (samples from different sources) such as Wounds, blood, urine and sputum (from Milad Hospital) in January 2019 to May 2020. The steady development of antibiotic resistance in various bacteria poses a high-risk threat to the health of the global community. Activation of quorum sensing signaling and biofilm formation leads to antimicrobial resistance of pathogens, thus increasing the difficulty in treating bacterial diseases. In this study, 50 clinical samples of patients were prepared in Milad Hospital, Tehran. By confirmatory and differential tests, these samples were confirmed as strains of Pseudomonas aeruginosa. After obtaining the MIC of iron oxide nanoparticles, this concentration (MIC = 0.512) of the nanoparticles was applied to the bacteria. The effect of iron-oxide surface nanoparticles on bacterial pathogens and genes affecting it (LasI and LasR) was investigated. Therefore, in this study, the expression of LasR and LasI genes in quorum sensing system under the influence of iron oxide nanoparticles modified surface structure with silver was investigated in the clinical strain of Pseudomonas aeruginosa.The analysis of data revealed that In microbiological (phenotypic) studies, the effect of iron oxide nanoparticles on surface structure modified with silver led to the lack of growth of Pseudomonas aeruginosa. The expression of LasR and LasI genes was significantly reduced under the influence of iron oxide nanoparticles modified surface structure with silver.In this study, the fidings that iron oxide nanoparticles modified by surface structure can be effective on preventing pathogens by Pseudomonas aeruginosa. Also, surface iron oxide modified silver nanoparticles have antimicrobial properties in inhibiting bacterial growth there are various that are applied through different mechanisms. Decreased expression of LasR and LasI genes causes our nanoparticles to inhibit the quorum sensing system. Therefore, this study proposes to investigate the synergistic effect of iron oxide nanoparticles with antibiotics and other genes from the quorum sensing system.
Citation: Mohammad Ali Zamirian1, Shohreh Zare Kariz (2022) Expression of LasR and LasI genes in quorum sensing system under the influence of iron oxide nanoparticles modified with silver surface structure in the clinical strain of Pseudomonas aeruginosa, European Journal of Biology and Medical Science Research, Vol.10, No.2, pp.45-61
Keywords: Biofilm, Iron oxide nanoparticles Modified surface structure with silver, LasI gene, LasR gene, Pseudomonas aeruginosa, Quorum Sensing
