Oxidative stress is involved in aging and many diseases, such as neurodegenerative, psychiatric disorders, and various cancers. Recyling oil contains trans fat (TF), one of sources of oxidative stress induce lipid peroxidation resulting in malondialdehyde (MDA) and 4-hydroxynonenal (HNE) generation. Green tea has gained considerable attention because of its antioxidant features. This study focuses on the effect of green tea on plasma MDA levels as one of marker for oxidative stress among wistar fed by recycling canola oil. A post test only control group design study using wistar rat. Samples were randomly divided into five groups (n=5 per group). K1 (negative control), K2 was given recycling canola oil and standard diet for 4 weeks, K3 was given green tea and standard diet for 4 weeks, P1 was given green tea and recycling canola oil diet for 4 weeks and P2 was given recycling canola oil diet for 4 weeks then green tea and standard diet for 4 weeks. Blood samples were collected from abdominal aorta to measure plasma MDA levels using modification of TBARS methods as described by Yagi et al.
There is no significant difference between groups on body weight (p=0.310), abdominal circumference (p=0.503) and plasma MDA levels (p=0.398) after giving green tea to wistar fed by standard diet and supplemented by recycling canola oil. We conclude that giving green tea to wistar fed by standard diet and suplemented by recycling canola oil do not influence on body weight, abdominal circumference and plasma MDA levels.
Keywords: Malondialdehyde, Oxidative stress, Trans fat
