This study determined the effectiveness of demonstration, individualized and conventional methods and Gender on the achievement of Secondary School Two students in biology. The study employed a multi stage sampling techniques; in the first stage, three schools were randomly selected out of the twenty Secondary Schools in Abeokuta South Local Government of the state. At the second stage, sample sizes of sixty students were randomly selected from the list of students and their gender. In the same manner, thirty students were male while the female constitute the same number. The study which lasted six weeks made use of two valid and reliable instruments: Biology Achievement Test (BAT) and Operational guide for instruction (OGI) Stimulus instrument. Students were exposed to different methods (Demonstration and Individualized) while those in the control were not exposed to any treatment but were rather taught in the Convectional way. Data analysis involved the use of Analysis of Covariance, Descriptive Statistics and t-test.The result indicated that the demonstration method is a more potent method of improving achievement in biology. (F (1.167) = 42.838; P < 0.05), (F (1.167) = 486.287; P < 0.05), F (2.167) = 90.389; P < 0.05). Moreover, there was a significant difference in the academic achievement of male and female students when exposed to the two experimental methods and control, hence female students performed better than their male counterparts. Arising from these findings, demonstration method and individualized method where recommended for teachers use in biology classrooms.
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